基质金属蛋白酶在实验性变态反应性脑脊髓炎病理改变中的作用及意义

目的：研究实验性变态反应性脑脊髓炎（EAE）的病理变化及基质金属蛋白酶（MMPs）的表达，探讨MMPs在EAE病理改变中的作用及意义。方法：应用光镜、电镜观察EAE大鼠模型组织学改变，利用免疫组化方法研究MMP-2、-9的表达。结果：光镜下可见小血管周围炎细胞浸润，呈袖套状改变，血管周围明显脱髓鞘，电镜下可见髓鞘松散、断裂，轴索细胞器消失，神经元内质网扩张脱颗粒。免疫组化染色MMP-2、-9在脊膜细胞、炎细胞和内皮细胞内均呈阳性表达。结论：MMPs参与EAE发病的各个环节，具有破坏血脑屏障、降解髓鞘、损伤轴索和产生免疫原的作用。

The effect and the significance of the matrix metalloproteinases in the pathological changes of the rat experimental allergic encephalomyelitis

Objective:To investigate pathological changes and the expression of the matrix metalloproteinases in rat EAE.Methods:The pathological changes of EAE were studied in Wistar rat with the aid of light and electron microscope and the expression and distribution of MMP-2,MMP-9 in different tissues were detected with the method of immunohistochemistry.Results:Light microscopy showed inflammatory cuff around small blood vessels were evident, disintegration and disappear of myelin sheath were observed. Electron microscopy showed a lot of loose and fragmental spiremes of myelin sheath, the component of axons and nissl bodies in neurons were disappeared. MMP-2,-9 were expressed intensively in vascular endothelial cells, meninges and accumulative inflammatory cells.Conclusion:MMPs take the roles in every aspect of the pathological changes in EAE, it can destroy the blood brain-barrier, degrade the myeline sheath, damage the axons and generate immunogens.