| 猪血红细胞膜中鞘磷脂降解成神经酰胺的实验研究 |
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| 引用本文： | 周淡宜,徐水祥,唐靓.猪血红细胞膜中鞘磷脂降解成神经酰胺的实验研究[J].中国现代应用药学,2004,21(3):169-170. |
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| 作者姓名： | 周淡宜 徐水祥 唐靓 |
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| 作者单位： | 浙江省医学科学院,浙江,杭州,310013 |
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| 摘 要： | 目的从红细胞膜中提取鞘磷脂并降解成神经酰胺.方法分离猪血细胞,用溶血,脱水,干燥等方法提取鞘磷脂,并用磷脂酶C降解鞘磷脂成神经酰胺.结果用此法可提取到红细胞膜中的鞘磷脂,并最后获得神经酰胺.结论用此法可将红细胞膜中的鞘磷脂降解成神经酰胺.
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| 关 键 词： | 红细胞膜 鞘磷脂 提取 磷脂酶C 神经酰胺 |
| 文章编号： | 1007-7693(2004)03-0169-02 |
| 修稿时间： | 2003年1月14日 |
Study on the hydrolyzation of the sphingomyelin from pig erythrocyte membrane to ceramide |
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| ZHOU Dan-yi,XU Shui-xiang,TANG Liang.Study on the hydrolyzation of the sphingomyelin from pig erythrocyte membrane to ceramide[J].The Chinese Journal of Modern Applied Pharmacy,2004,21(3):169-170. |
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| Authors: | ZHOU Dan-yi XU Shui-xiang TANG Liang |
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| Abstract: | OBJECTIVE To extract the sphingomyelin from erythrocyte membrane and hydrolyze it to ceramide. METHOD Sphingomyelin was prepared through the process of separating erythrocyte from blood,erythrocytolysis,dehydration,dry,et al,and then hydrolyzed to ceramide by phospholipase C. RESULTS The sphingomyelin was obtained from erythrocyte membrane with this method and the ceramide was consequently obtained. CONCLUSION Sphingomyelin can be obtained from erythrocyte membrane by this method. |
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| Keywords: | erythrocyte membrane sphingomyelin extraction phospholipase C ceramide |
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