应用氟尿嘧啶治疗胃癌患者预后与其癌组织中尿嘧啶核苷磷酸化酶基因的高表达相关性研究

目的:分析尿嘧啶核苷磷酸化酶基因表达水平与胃癌、肺癌和结肠癌的预后相关性。方法:采用RT-PCR法检测了28例胃癌、14例肺癌和22例结肠癌组织及其远癌正常组织中尿嘧啶核苷磷酸化酶(UP)基因的表达。结果:对28例胃癌组织及其远癌正常组织检测发现,有20例UP基因的表达比正常组织增高,另8例UP基因的表达比正常组织低或二者相等。所有这些胃癌患者均在手术前、后接受了氟尿嘧啶的治疗。胃癌组织中UP基因表达增高的患者比UP基因表达正常或降低的患者有较好的生存率;相反,大多数的结肠癌(16例)和所有的肺癌患者未接受氟尿嘧啶的治疗,且大部分的结肠癌(12例)和肺癌(10例)样本显示癌组织中UP基因呈低表达。肺癌和结肠癌组织相对于其远癌正常组织中UP基因的不同表达与患者的生存率不相关。结论:胃癌组织相对于其远癌正常组织的UP基因的表达水平可作为判断接受氟尿嘧啶的治疗的胃癌患者预后的一个指标。

Dependability between Upregulated Expression of the Uridine Phosphorylase Gene in Gastric Cancer Tissues and Favorable Prognosis of the Patients Treated by PFMT

Objective:To investigate the relationship between the expression levels of uridine phosphorylase(UP)gene and the prognosis of patients with the gastric,colon and lung carcinomas.Methods:The mRNA level of UP gene was examined using RT-PCR in 28 cases of stomach carcinoma,14 lung cancer and 22 colon carcinoma.Results:Among the 28 paired gastric samples,20 showed higher expression level in tumors than in normal surrounding tissues and 8 cases showed equal or lower levels in the tumors.All the gastric patients received 5-Fu before and/or after the surgical resection and the prognosis of the patients whose UP gene expression in tumors was increased was relatively better than that with equal or less UP gene expression in the tumors.In contrast,most of patients with colon carcinoma(16 cases)and all with lung cancer(14 in total)did not receive 5-Fu and majority of the colon(12 cases)and lung(10 cases)specimens showed a lower expression in the cancerous region.The differential expression between the cancerous and non-cancerous regions in the patients with colon and lung cancer was not related with the prognosis.Conclusion:These data suggest that the paired expression level of UP gene in gastric cancer is a possible prognostic marker for the patients who received 5-Fu.