IL-6对人系膜细胞c-myc原癌基因表达的影响以及大黄素的拮抗作用

本文应用体外细胞培养和斑点杂交技术,首次对重组人IL-6对人系膜细胞c-myc原癌基因mRNA的表达以及大黄素对IL-6的作用进行了观察。发现IL-6能够促进人系膜细胞c-myc原癌基因mRNA的增殖表达,该作用呈明显的剂量依赖性,而大黄素能够拮抗IL-6的上述作用。本项研究阐明了IL-6促进系膜细胞的可能机理,为进一步论证大黄素对系膜细胞功能的影响,大黄素与细胞因子间的相互作用以及大黄在防治肾小球硬化的作用奠定了基础。

Effect of IL-6 on c-myc protooncogene expression in human mesangial cells and the down-regulation by Emodin

To investigate further the possible molecular mechanism of IL-6 on mesangial cell proliferation, we examined the effect of IL-6 on c-myc protooncogene mRNA expression in cultured human mesangial cells (MC)by dot blot hybridization. Mesangial cells(2 x10~6)made quiescent by exposed to a defined serum-free medium were used in the experiment. IL-6 and Emodin were added ndependently or together to the media of cultured human MC at zero time. It was found that the c-myc mRNA expressed was enhanced markedly in MC treated with IL-6. This effect was dose-dependent and blocked by Emodin. In conclusion: (1) In quiescent MC, a low basal xpression of c-myc mRNA could be detected. (2) Exposure of MC to IL-6 resulted in a marked increasement of c-myc mRNA expression in adose-dependent manner. This sequential activation of protooncogene and cellular reaction may be an important mechanism in IL-6 induced MC proliferation. (3) Emodin has down-regulating effect on c-myc mRNA overexpression of MC induced by IL-6. It is speculated that Emodin might inhibit MCproliferation through this path way.