太原地区HBV宫内传播的基因型分布及传播特征

目的　了解太原地区乙型肝炎病毒 (HBV)感染孕妇及新生儿HBV基因型分布及宫内传播特征。方法　采集HBsAg阳性孕妇及其新生儿外周血 ,无菌分离血清采用巢式聚合酶链反应 -限制性片段长度多态性 (nPCR RFLP)方法鉴定HBV基因型。结果　 16 2例HBsAg阳性孕妇中 ,HBVDNA阳性 6 3例 ,其中B基因型 5例 (7.9% ) ,C基因型 5 0例 (79.4 % ) ,未能分型 8例 (12 .7% ) ;16 4例 (2对双胎 )新生儿中HBVDNA阳性 2 4例 ,其中B基因型 1例 (4.2 % ) ,C基因型 2 1例 (87.5 % ) ,B、C混合型 1例 (4.2 % ) ,未能分型 1例 (4.2 % )。新生儿HBV基因型与母亲基本一致。结论　太原地区HBV感染的孕妇及新生儿中 ,C基因型占绝对优势 ,宫内传播过程中HBV基因型基本保持不变。

Distribution of hepatitis B virus genotypes in intrauterine transmission and characteristics in Taiyuan

Objective To study the distribution of hepatitis B virus(HBV) genotypes of mothers and their newborns in Taiyuan and the characteristics of intrauterine transmission. Methods HBV genotype was determined by nested-polymerase chain reaction(nPCR) and restriction fragment length polymorphism (RFLP) analysis. Results Of 162 HBsAg positive mothers,63 were HBV DNA positive, 5(7.9%) were genotype B, 50(79.4%) were genotype C, and 8(12.7%) were uncertain. Of 164 newborns, 24 were HBV DNA positive, 1(4.2%) were genotype B, 21(87.5%) were genotype C, 1(4.2%) was infected with both B and C, and 1(4.27%) was uncertain.The genotype of HBV in newborns and their mothers was consistent. Conclusion Genotype C was the major genotype in intrauterine transmission in Taiyuan.Genotype of HBV was not changed in intrauterine transmission.