| RDP1258对大鼠脾细胞增殖及HO-1活性的抑制作用 |
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| 引用本文： | 易善红,宋波,汪泽厚,张艮甫,崔志刚. RDP1258对大鼠脾细胞增殖及HO-1活性的抑制作用[J]. 细胞与分子免疫学杂志, 2004, 20(5): 632-634 |
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| 作者姓名： | 易善红 宋波 汪泽厚 张艮甫 崔志刚 |
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| 作者单位： | 第三军医大学附属新桥医院泌尿外科,重庆,400037 |
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| 基金项目： | 国家新药基金资助项目 (No.1 999A0 0 2 ) |
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| 摘 要： | 目的 :观察一种新型HLA衍生肽 (RDP12 5 8)的免疫抑制作用 ,并探讨其机制。方法 :用化学方法合成一种HLA衍生肽(RDP12 5 8) ,以3 H TdR掺入法观察其在体外对大鼠脾细胞增殖的影响 ;以酶化学法观察其对脾细胞血红素氧合酶 1(hemeoxygenase 1,HO 1)活性的影响。结果 :RDP12 5 8可显著抑制大鼠脾细胞增殖并以剂量依赖的方式降低HO 1的活性。结论 :RDP12 5 8能够显著抑制丝裂原或同种抗原刺激所致大鼠脾细胞的增殖反应 ,其抑制作用可能是通过降低HO 1活性而实现的
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| 关 键 词： | HLA衍生肽 混合淋巴细胞培养 血红素氧化酶-1 |
| 文章编号： | 1007-8738(2004)05-0632-03 |
| 修稿时间： | 2003-09-17 |
Inhibitory effects of HLA-derived peptide RDP1258 on proliferation and heme oxygenase-1 activity of rat splenocytes |
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| YI Shan-hong,SONG Bo,WANG Ze-hou,ZHANG Gen-fu,CUI Zhi-gang. Inhibitory effects of HLA-derived peptide RDP1258 on proliferation and heme oxygenase-1 activity of rat splenocytes[J]. Chinese journal of cellular and molecular immunology, 2004, 20(5): 632-634 |
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| Authors: | YI Shan-hong SONG Bo WANG Ze-hou ZHANG Gen-fu CUI Zhi-gang |
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| Affiliation: | Department of Urology, Xingqiao Hospital, Third Military Medical University, Chongqing, 400037, China. yshh881@sohu.com |
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| Abstract: | AIM: To observe the immunosuppression effect of a novel HLA-derived peptide RDP1258, and to explore its mechanisms. METHODS: RDP1258 was synthesized chemically. The effect of the peptide on alloreactive proliferation of rat splenocytes was observed by 3H-TdR incorporation method. The heme oxygenase-1(HO-1) activity was analyzed by the enzyme assay. RESULTS: The results showed that the synthetic HLA-derived peptide could obviously inhibit the proliferation of rat splenocytes and HO-1 activty in a dose-dependment manner in vitro. CONCLUSION: HO-1 may participate in the inhibitory effect of RDP1258 on the proliferation of rat splenocytes induced by mitogen or isoantigen. |
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| Keywords: | HLA-derived peptide mixed leukocyte culture heme oxygenase-1 |
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