| 汽车安全气囊上微量检材DNA检测方法的探讨 |
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| 引用本文： | 董雪梅,郭刚,陈立方,王小梅,许冰莹. 汽车安全气囊上微量检材DNA检测方法的探讨[J]. 昆明医学院学报, 2011, 32(6): 49-52 |
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| 作者姓名： | 董雪梅 郭刚 陈立方 王小梅 许冰莹 |
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| 作者单位： | 1. 昆明医学院法医学院,云南昆明,650500
2. 云南省公安厅刑侦总队,云南昆明,650000 |
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| 基金项目： | 云南省应用基础研究基金资助项目，云南省科技厅-昆明医学院联合专项基金资助项目 |
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| 摘 要： | 目的探讨汽车安全气囊上微量人体脱落上皮细胞DNA检测的有效方法.方法分别采用棉线载体直接扩增法、小体积Chelex-100法和磁珠法对汽车安全气囊上提取的微量人体脱落上皮细胞的DNA进行检测,并对检测结果进行分析比较,以获得有效方法.结果棉线载体直接扩增法和磁珠法均能得到满意的人体脱落上皮细胞核DNA STR基因座分型结果,小体积Chelex-100法不能获得STR基因座分型结果.结论棉线载体直接扩增法和磁珠法均能有效地检测微量人体DNA,获得STR基因座分型结果,提高DNA检出率,操作简单、节约时间和试剂成本,特点在法医物证学实际检案工作中具有较高的应用价值.
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| 关 键 词： | 汽车安全气囊 微量人体DNA检测方法 STR基因座 个人识别 法医学应用 |
Trace DNA Detection Methods in Airbag of Vehicle |
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| DONG Xue -mei,GUO Gang,CHEN Li-fang,WANG Xiao -mei,XU Bing-ying. Trace DNA Detection Methods in Airbag of Vehicle[J]. Journal of Kunming Medical College, 2011, 32(6): 49-52 |
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| Authors: | DONG Xue -mei GUO Gang CHEN Li-fang WANG Xiao -mei XU Bing-ying |
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| Affiliation: | DONG Xue-mei1),GUO Gang1),CHEN Li-fang2),WANG Xiao-mei1),XU Bing-ying1)(1)School of Forensic Medicine,Kunming Medical University,Kunming Yunnan 650031,2)Yunnan Provincial Public Security Bureau,Kunming Yunnan 650000,China) |
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| Abstract: | Objective To discuss the effective DNA detection method for the trace of human epithelial cells exfoliated on airbag of vehicle.Methods The trace DNA of human epithelial cells extracted form the airbag was detected by the methods of cotton carrier direct amplification,small volume of Chelex-100,and the detection of magnetic beads,and the results were analyzed and compared in order to obtain a valid method.Results Cotton carrier direct amplification method and the detection of magnetic beads method can get t... |
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| Keywords: | Airbag Trace DNA detection of human STR loci Personal identification Forensic medicine application |
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