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弥散性血管内凝血的放射自显影研究
引用本文:孙去病,肖家声,陈广文. 弥散性血管内凝血的放射自显影研究[J]. 中国病理生理杂志, 1988, 0(2)
作者姓名:孙去病  肖家声  陈广文
作者单位:湖南医科大学病理生理教研室(孙去病,肖家声),湖南医科大学病理生理教研室(陈广文)
摘    要:大白鼠注射~(125)I标记的纤维蛋白原后,再注射凝血酶,造成弥散性血管内凝血。放射自显影检查发现,同位素标记的微血栓主要位于肾小球毛细血管,直小血管之中。肺泡的毛细血管也可发现这些微血栓。肝脏和脾脏的网状内皮细胞吞噬了大量的标记物质,这些吞噬细胞定位在肝门区,而在脾脏则位于马氏小体周围。此外,在肾小管的上皮细胞内也发现有同位素,这些物质可能是通过肾小球过滤而被肾小管重吸收的标记物质。用自显影术,常规染色和纤维蛋白的特殊染色互相比较,肺的微血栓在常规和特殊染色中不能被发现,用自显影术则很易检出同位素标记的微血栓。

关 键 词:弥散性血管内凝血  放射自显影术  网状内皮系统

Preliminary autoradiographic research for disseminated intravascular coagulation
Sun Qu-Bing,et al. Preliminary autoradiographic research for disseminated intravascular coagulation[J]. Chinese Journal of Pathophysiology, 1988, 0(2)
Authors:Sun Qu-Bing  et al
Affiliation:Sun Qu-Bing,et al Department of Pathophysiology,Hunan Medical University,Changsha
Abstract:Disseminated intravascular coagulation was induced by injection of thrombin and EACA in rats.In the experimental group the rats, after injection of ~(125)I fibrinogen, received thrombin to produce DIC. The control group received saline instead of thrombin. The autoradiographic result indicated that the radioactive microthrombi were mainly situated in the glomeruli of kidney and pulmonary capillaries. By the use of HE and PTAH stain, however, the microthrombi could be found only in the glomeruli of the kidney but not in pulmonary capillaries. This indicated that autoradiography was more sensitive than histochemical and HE methods for detection of thrombi in microcirculation. It was also discovered that the reticuloendothelial cells of liver and spleen phagocytized a large amount of the radioactive materials. The materials might be radioactive fibrin or its monomer because ~(125)I fibrinogen has been attached to thrombin. In addition to these, the epithelium of convoluded tubules of kidney also absorb radioactive materials which might be peptides A and B.
Keywords:Disseminated intravascular coagulation  Autoradiography  Reticuloendothelial system
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