| Periostin对人牙周膜细胞增殖和碱性磷酸酶活性的影响 |
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| 引用本文： | 万玲,吴织芬,刘斌,宋应亮,袁乃梅.Periostin对人牙周膜细胞增殖和碱性磷酸酶活性的影响[J].口腔医学研究,2001,17(1):17-19. |
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| 作者姓名： | 万玲 吴织芬 刘斌 宋应亮 袁乃梅 |
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| 作者单位： | 第四军医大学口腔医学院 |
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| 基金项目： | 国家自然科学基金资助 !(编号 :3 970 0 164 ) |
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| 摘 要： | 目的 :研究小鼠重组Periostin对牙周膜细胞 (PDL细胞 )增殖及碱性磷酸酶 (ALP)活性的影响。方法 :采用MTT比色试验及酶动力学方法 ,测定PDL细胞的增殖和ALP活性。结果 :Periostin仅在浓度为 40 μg/ml时 ,可显著促进PDL细胞的增殖 (P <0 .0 1) ,其余浓度则无作用 ,而其浓度在 10 μg/ml～ 80 μg/ml范围中时 ,均可显著提高PDL细胞ALP活性水平 (P <0 .0 5 )。结论 :小鼠重组Periostin对PDL细胞的增殖及ALP活性均有促进作用 ,但其在这两方面的作用存在差异 ,对此还需深入研究
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| 关 键 词： | 细胞粘附分子 牙周膜细胞 噻唑盐 碱性磷酸酶 |
| 文章编号： | 1000—4661(2001)01—0017—03 |
| 修稿时间： | 2000年9月27日 |
The effects of mouse recombinant Periostin on proliferation and ALP activity of PDL cells. |
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| WAN Ling,WU Zhi fen,LIU Bin,et al..The effects of mouse recombinant Periostin on proliferation and ALP activity of PDL cells.[J].Journal of Oral Science Research,2001,17(1):17-19. |
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| Authors: | WAN Ling WU Zhi fen LIU Bin |
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| Institution: | WAN Ling,WU Zhi fen,LIU Bin,et al.Department of Periodontology,School of Stomatology,Fourth Military Medical University,Xi'an 710032 [ |
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| Abstract: | Objective:To investigate the effects of mouse recombinantPeriostin on proliferation and ALP activity of periodontal ligament cells (PDL cells).Methods:MTT method and enzyme dynamics method were used to observe the ability of proliferation and ALP expression of PDL cells.Results:Significant proliferation of PDL cells could be observed on Periostin at dose 40ug/ml (P<0.01) and the ALP activity was increased at dose10μg/ml～80μg/ml(P<0.05).Conclusion:Periostin can promote the proliferation and ALP activity of PDL cells,but the effects need further studies. |
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| Keywords: | Cell adhesion molecule Periodontal ligament cells MTT ALP |
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