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| 手工微柱凝胶免疫检验法在ABO、RhD血型抗原鉴定中的应用 | |
| 引用本文： | 郭博,马华,李兴武,杨清平. 手工微柱凝胶免疫检验法在ABO、RhD血型抗原鉴定中的应用[J]. 蚌埠医学院学报, 2005, 30(1): 72-73 |
| 作者姓名： | 郭博 马华 李兴武 杨清平 |
| 作者单位： | 1. 蚌埠医学院附属医院输血科, 安徽蚌埠 233004;2. 蚌埠医学院免疫学教研室, 安徽蚌埠 233003;3. 蚌埠医学院附属医院检验科, 安徽蚌埠 233004;4. 安徽省蚌埠市中心血站, 233000 |
| 摘 要： | 目的: 探讨手工微柱凝胶免疫检验法在ABO、RhD血型抗原鉴定中的应用。方法: 分别取50份不同科室、病种、性别的患者新鲜抗凝、非抗凝血标本为Ⅰ、Ⅱ组;取50份库存15天以上有效期内的库存血标本为Ⅲ组;取50份保存3~7天的非新鲜血标本(可能被污染)为Ⅳ组,同时用手工微柱凝胶法和试管法进行ABO、RhD血型抗原鉴定,比较反应结果及反应强度。结果: Ⅰ、Ⅱ、Ⅲ组标本在ABO、RhD抗原鉴定中,手工微柱凝胶法与试管法所得结果完全相符(P>0.05),反应强度微柱凝胶法高于试管法;Ⅳ组标本在ABO、RhD血型抗原鉴定时微柱凝胶法检出2例假阳性标本,其余48份标本两法鉴定结果完全相符(P>0.05)。结论: 手工微柱凝胶法可代替试管法常规用于ABO、RhD血型抗原鉴定。 |
| 关 键 词： | 血型鉴定和交叉配血 手工微柱凝胶法 试管法 |
| 文章编号： | 1000-2200(2005)01-0072-02 |
| 收稿时间： | 2004-02-27 |
| 修稿时间： | 2004-02-27 |
Evaluation of manual microcolumn gel immune method for detecting antigens of blood groups of ABO and RhD |
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| Affiliation: | 1. Department of Blood Transfusion, Affiliated Hospital of Bengbu Medical College, Anhui 233004;2. Department of Immunology, Bengbu Medical College, Anhui 233003;3. Department of Clinical Laboratories, Affiliated Hospital of Bengbu Medical College, Anhui 233004;4. Bengbu Blood Center, Anhui 233000, China |
| Abstract: | Objective: To evaluate manual microcolumn gel immune method in detecting antigens of blood groups of ABO and RhD.Methods: Fifty samples of fresh anticoagulant blood,50 samples of fresh non-anticoagulant blood,50 samples of blood stocked for 15 days,but within valid period and 50 samples of non-fresh blood stocked for 3-5 days(possibly contaminated blood),which were collected from different departments,diseases and genders of patients,were classified into Ⅰ,Ⅱ,Ⅲ and Ⅳ groups respectively.Blood antigens of ABO,RhD in all samples were assayed by manual microcolumn gel immune method and tube method.The response result and response intension were compared.Results: The result by manual microcolumn gel immune method in Ⅰ,Ⅱ and Ⅲ groups was identical with that by tube method in detecting antigens of blood type of ABO and RhD(P>0.05),but the response intension was stronger by manual microcolumn gel immune method than that by tube method.Two improper positive samples in Ⅳ group were found by manual microcolumn gel immune method and the other 48 samples had the same results by two methods(P>0.05).Conclusions: Manual microcolumn gel immune method can substitute tube method for detecting antigens of blood groups of ABO and RhD. |
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