大蒜新素注射液抗鼠肝炎病毒3型的体外实验研究

目的：探讨大蒜新素（allitridin）在细胞水平对鼠肝炎病毒3型（mice hepatitis virus,MHV-3）的抑制作用.方法：细胞培养、蚀斑试验测定病毒滴度;MTT法进行药物毒性试验;蚀斑抑制试验检测大蒜新素的抗MHV-3活性,改变给药时间探索大蒜新素抗MHV-3作用环节.结果：大蒜新素半数中毒浓度（TC50）为75 mg/L,抗MHV-3病毒的半数有效浓度（IC50）为2.7 mg/L,治疗指数（TI）为27.78,大蒜新素对MHV-3病毒的抑制作用存在明显的量效反应关系（P＜0.01）,并且对MHV-3病毒有预防和中和作用（P＜0.01）.结论：大蒜新素在L2细胞中对MHV-3病毒有明显的抑制作用,其作用机制是多途径的.

In vitro antivirus effect of allitridin injection on hepatitis virus strain 3 in Mouse

Objective: To investigate the effect of allitridin injection on hepatitis virus strain 3(MHV-3) in mouse and the mechanism.Methods:A MHV-3 infected cell model was established by a MHV-3-infected L2 cell cultures.The maximum tolerance concentration(MTC) of L2 cell bearing to allitridin treatment was measured with MTT colorimetric assay.The median effective inhibitory concentration(IC50) of allitridin was determined by plaque-reduction assay.The antiviral effect of allitridin was studied by using allitridin with different administration procedures and routes.Results:The median toxic concentration(TC50) of allitridin was 75 mg/L,and the IC50 of allitridin was 2.7 mg/L.The treatmen index(TI) was 27.78.The MTC of allitridin was 7.6 mg/L.No matter what routes of allitridin was used in,the MHV-3 was effectively suppressed by allitridin treatment.Conclusion:Allitridin is an effective anti-MHV-3 medicine and has various antviral mechanism.