A simple microplate algal assay technique for aquatic toxicity assessment

A simple, miniaturized algal assay procedure using microplates has been developed to assess aquatic toxicity with the green alga, Selenastrum capricornutum. Cell count-generated EC₅₀ data comparisons with the classic assay using flasks have indicated good agreement between the two methods following toxic assessment of various wastewater samples and metal solutions. Parametric comparisons (ATP us cell counts) with the microplate method show equally good correlation. The technique is highly versatile in conducting basic algal bioassays for varying times (4-hour, 4-day, 8-day EC₅₀'s) and with differing parameters, depending on set objectives. Other interesting features of the microplate technique include handling rapidity, economy, space-saving convenience, and automation potential.