慢性低氧大鼠骨骼肌线粒体的蛋白质组学研究

目的： 采用蛋白质组学方法研究慢性低氧大鼠线粒体蛋白表达变化并讨论其病理生理学意义。方法： Wistar大鼠随机分为2组：慢性低氧组和对照组。动物麻醉后取双侧腓肠肌标本，分离纯化线粒体蛋白进行二维电泳和ImageMaster分析，对差异蛋白质用MALDI-TOF-MS质谱仪进行肽指纹图谱测定。结果： 建立了大鼠腓肠肌线粒体蛋白质二维凝胶图像；与对照组相比，慢性低氧组大鼠线粒体350-450个蛋白点中共有35个蛋白表达显著改变，其中27个表达下调，8个表达上调；并对低氧相关的线粒体蛋白进行了鉴定。结论： 慢性低氧可诱导低氧相关线粒体蛋白表达改变，其作用可能与线粒体氧化磷酸化、物质转运和脂代谢有关。

Proteomic study on skeletal muscle mitochondrial proteins of chronic hypoxic Wistar rats

AIM: To explore hypoxia-related mitochondrial proteins in Wistar rats exposed to chronic hypobaric hypoxia.METHODS: Adult male Wistar rats were randomized into chronic hypoxia (30 d) group and control group. Bilateral gastrocnemius were collected and mitochondria were extracted and purified for two dimensional electrophoresis (2-DE) and matrix assisted laser desorption/ionization mass spectrometry (MALDI-TOF-MS) analysis.RESULTS: Compared to the control, 35 protein spots in chronic hypoxia group were up-regulated (8 protein spots) or down-regulated (27 protein spots), 8 of which were identified as F1-ATPase alpha subunit, voltage-dependent anion channel 1, androgen regulated protein, tripartite motif protein 50, hydroxyacyl-coenzyme A dehydrogenase alpha subunit and mitochondrial F1 complex gamma subunit.CONCLUSION: Our results suggest that hypoxia exposure may affect mitochondrial electron transport chain, oxidative phosphorylation and lipid metabolism.