间日疟原虫抗原体外诱导间日疟患者PBMC凋亡的研究

目的分析间日疟原虫抗原(PvAg)诱导宿主外周血单个核细胞(PBMC)的凋亡水平方法采用流式细胞术结合应用膜联蛋白-V(Annexin-V),测定7例间日疟现症感染者PBMC经PvAg刺激后CD4+T细胞、CD8+T细胞及CD19+B细胞的凋亡率,并与空白对照组比较。结果经PvAg刺激后的间日疟现症感染者CD19+B细胞及CD4+T细胞凋亡率分别为7.26%和48.08%,与空白对照凋亡率4.08%和22.80%比较差异无统计学意义(P>0.05);CD8+T细胞凋亡率为55.16%,与对照凋亡率22.86%比较差异有统计学意义(P<0.05)。结论 PvAg可诱导间日疟现症感染者CD8+T细胞凋亡,从而可能参与介导间日疟原虫发生免疫逃逸。

Apoptosis of PBMCs induced in vitro by Plasmodium vivax antigen in patients with vivax malaria

Objective To investigate the level of PBMC apoptosis induced by Plasmodium vivax crude antigen(PvAg)during acute P.vivax infection.Methods PBMCs from 7 patients infected with P.vivax were incubated with PvAg.Next,the percentage of apoptotic CD4+T cells,CD8+T cells,and CD19+ B cells was determined using flow cytometry(FCM) with annexin V and corresponding CD3 McAb.Results The percentage of apoptotic CD3+CD8+ T cells in PvAg-stimulated cells(55.16%) was significantly higher than that with spontaneous apoptosis observed in cultured cells without any stimulus(22.86%,P<0.05),but there were no significant differences in the percentages of apoptotic CD19+ B cells and CD4+T cells between PvAg-stimulated cells(7.26% and 48.08%,respectively) and spontaneously apoptotic cells(4.08% and 22.80%,respectively,P>0.05).Conclusion P.vivax induced the apoptosis of CD8+ T cells,which may be partly responsible for the parasite’s evasion of the immune response.