豆腐果苷对慢性应激小鼠海马神经元再生的影响

 目的 研究豆腐果苷对慢性应激小鼠海马神经元再生的影响，探讨其抗抑郁作用的可能机制。方法 给予小鼠多种慢性不可预知性应激刺激，建立小鼠抑郁模型，刺激前30 min给予豆腐果苷（10、20、40 mg·kg^-1，ig），连续28 d。用免疫组化实验检测海马齿状回神经前体细胞分裂及脑源性神经营养因子（BDNF）表达，Western蛋白印迹法检测海马BDNF蛋白表达水平。结果 免疫组化显示，慢性应激28 d小鼠海马齿状回神经前体细胞分裂减少，同时BDNF水平低下，均表现为阳性棕色颗粒缺失，豆腐果苷或盐酸氟西汀则明显增加神经前体细胞的分裂，并上调BDNF蛋白表达，提高BDNF水平。结论 豆腐果苷对慢性应激小鼠的抗抑郁作用机制可能与促进海马齿状回神经前体细胞增殖，提高BDNF水平有关。

Effects of Helicid on Hippocampal Neurogenesis in Chronically Stressed Mice

OBJECTIVE To explore the effects of helicid on the hippocampal neurogenesis in chronically stressed mice and its possible action mechanism of antidepressants.METHODS Mice were exposed to chronic stress and isolated breed for 28 consecutive days with coadministration of helicid(10,20,40 mg·kg-1,ig) or fluoxetine(2.6 mg·kg-1,ig).The proliferation of hippocampal progenitor cells and level of brain derived neurotrophic factor(BDNF) were measured by immunohistochemistry and Western blot analysis.RESULTS Immunohistochemistry showed decreased expression of BrdU and BDNF in hippocampal dentate gyrus in chronic stress mice.Compared with chromic stress group,helicid and fluoxetine significantly increased expression of BrdU and BDNF.CONCLUSION The antidepressant mechanisms of Helicid in chronically stressed mice is probalblely related to promote neurogenesis in dentate gyrus and induce up-regulation of BDNF.