藏族药小檗皮不同药用部位中4种生物碱成分的HPLC含量测定

目的:建立同时测定藏族药小檗皮药材中木兰花碱、药根碱、巴马汀、小檗碱含量的方法,并比较茎内皮、茎木、茎栓皮、根内皮、根木和根栓皮中4种成分的差异。方法:采用HPLC法,应用Inert Sustain C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.2%磷酸溶液,检测波长270 nm。结果:小檗皮不同部位之间的生物碱成分含量有明显的差异,根内皮及茎内皮中4种成分的含量明显高于茎木、茎栓皮、根木和根栓皮。此外,茎内皮比根内皮含有更多的木兰花碱,但小檗碱的含量明显低于根内皮,而两药用部位之间的总生物碱含量没有显著性差异。结论:该方法简便、准确、重复性好,可用于小檗皮药材的质量评控。从环境保护和可持续利用角度考虑,建议小檗皮药材取消根内皮而只以茎内皮入药。

Simultaneous Determination of Four Alkaloids in Different Medicinal Parts of Berberidis Cortex by HPLC

Objective: To establish an HPLC method for simultaneous determination of magnoflorine, jatrorrhizine, palmatine and berberine in Berberidis Cortex and compare the differences of the four alkaloids among different parts. Method: The separation was performed on an InertSustain C₁₈ column with a gradient elution of acetonitrile and 0.2% phosphoric acid solution, and the detection wavelength was set at 270 nm. Result: There were significant differences in the contents of four alkaloids among different parts of Berberidis Cortex. The contents of four alkaloids in root endodermis and stem endodermis were obviously higher than stem xylem, stem phellem, root xylem, and root phellem. Moreover, compared with root endodermis, stem endodermis contained more magnoflorine but less berberine. However, there was no significant difference in total alkaloid contents between the two medicinal parts. Conclusion: The established method was simple, accurate and repeatable, which was suitable for quality evaluation and quality control of Berberidis Cortex. Based on these findings, we suggest that the stem endodermis of Berberidis Cortex may be considered as the only medicinal part in perspective of environmental protection and sustainable utilization.