南充市无偿献血者 ABO 定型困难原因分析

目的:分析南充市无偿献血者ABO定型困难的原因,探讨ABO定型困难献血者所献血液的处理措施。方法:应用ABO正反定型、吸收放散试验、唾液血型物质测定、不规则抗体筛选鉴定等血型血清学方法,检测南充市红十字中心血站42名无偿献血者ABO定型困难的血液标本。结果:在本地42名无偿献血者ABO定型困难原因调查中发现ABO抗体减弱或消失26例(61.90%);ABO亚型13例(30.96%);单独血浆中存在不规则抗体3例(7.14%)。结论:造成本地无偿献血者ABO定型困难的主要原因是ABO抗体减弱或消失,其次为ABO亚型和单独血浆中存在不规则抗体,须依据各自情况对该类献血者所献血液作相应的处理,从而提高此类献血者所献血液临床应用的安全性和有效性,避免血液的浪费。

Analysis on the causes of ABO group typing difficulties in voluntary blood donors of Nanchong

Objective:To analyze the causes of difficulties in ABO blood group typing in unpaid donors of Nanchong and to dis-cuss the measures for the blood of these donors.Methods:Serological tests including ABO group forward and reverse typing,absorption-elution testing,determination on blood group materials in saliva,and screening on irregular antibody were adopted,to test blood speci-mens from 42 cases of unpaid donors from Nanchong Red Cross Central Blood Station,which were difficult in ABO blood group typing. Results:Cause analysis in 42 cases of local unpaid donors with difficulty in ABO blood group typing found that ABO antibodies were weakened or disappeared in 26 cases (61 .90%),there were ABO subtypes in 13 cases (30.96%),and there were irregular antibodies in plasma of 3 cases (7.14%).Conclusion:The main cause of difficulty in ABO blood group typing in local unpaid donors is reduction or disappearance of ABO antibodies,followed by ABO subtypes and the irregular antibodies in plasma alone.Different measures will be taken according to the specific circumstances,so as to improve the effectiveness and safety of clinical application of blood from these do-nors to avoid the waste of blood.