苦参-甘草药对提取工艺的优化及其化学成分分析

目的:优选苦参-甘草药对的提取工艺,并对其提取物的化学成分进行分析。方法:以苦参碱、氧化苦参碱和甘草酸的转移率为指标,在单因素试验基础上,采用正交试验考察乙醇体积分数、提取次数、提取时间和液料比对苦参-甘草药对提取工艺的影响。利用UPLC-Q-TOF/MS对苦参-甘草药对提取物化学成分进行在线鉴定。结果:苦参-甘草药对的最佳提取条件为加8倍量60%乙醇回流提取2次,每次2 h,提取前浸泡1 h;苦参碱和氧化苦参碱总转移率93.92%,甘草酸转移率99.23%。苦参-甘草药对提取物共鉴定出49个成分,其中28个来自于苦参,21个来自于甘草。结论:优选的提取工艺稳定可行。苦参-甘草药对配伍提取具有相互促进溶出的作用,为阐明其他药对的药效物质基础及配伍机制提供参考。

Optimization of Extraction Process for Couplet Medicines of Sophorae Flavescentis Radix-Glycyrrhizae Radix et Rhizoma and Identification of Its Chemical Constituents

Objective: To optimize extraction technology of couplet medicines of Sophorae Flavescentis Radix-Glycyrrhizae Radix et Rhizoma and to quickly identify the chemical constituents in its extract. Method: Taking transfer rates of matrine,oxymatrine and glycyrrhizic acid as comprehensive evaluation index,based on single factor tests,optimum extraction conditions of couplet medicines extract were selected by orthogonal test.The chemical constituents of couplet medicines extract were identified on-line by UPLC-Q-TOF/MS. Result: Optimum reflux extraction process was as follows:soaked for 1 h and extracted twice with 8 times the amount of 60% ethanol,2 h for each time.Total transfer rate of matrine and oxymatrine was 93.92%,the extraction yield of glycyrrhizic acid was 99.23%.Furthermore,a total of 49 constituents were identified in couplet medicines extract,28 constituents were from Sophorae Flavescentis Radix and 21 constituents were from Glycyrrhizae Radix et Rhizoma. Conclusion: The mixed extract has a certain auxo-action for extraction of matrine,oxymatrine and glycyrrhizic acid and the optimum extraction process is stable and feasible.Identification of chemical constituents in extract will be useful for elucidation of its active constituents and compatible mechanism.