雌性激素对妊娠豚鼠胆囊运动功能影响的实验研究

目的:探讨妊娠情况下雌性激素对豚鼠胆囊运动功能的影响及对胆囊结石形成的作用。方法:60只雌性豚鼠随机分为实验组(40只)和对照组(20只),实验组建立妊娠豚鼠动物模型,分为妊娠30d组和妊娠60d组,采用化学发光法和酶联亲和抗体组织化学法分别进行血清雌二醇(E2)、孕酮(Pg)浓度检测及胆囊组织雌激素受体(E R)、孕激素受体(PR)的检测,同时测量各组豚鼠胆囊空腹体积(FV),胆囊空腹胆汁量(FB)的变化,并观察胆囊结石形成情况。结果:妊娠60d组豚鼠有3只形成胆囊结石,妊娠豚鼠血清E2、Pg含量,胆囊组织E R、PR阳性表达率均明显高于非孕豚鼠(P<0.001),且随妊娠期进展而呈逐渐升高趋势。妊娠60d组豚鼠FV及FB明显大于妊娠30d组和对照组(P<0.001)。结论:妊娠期血清E2和Pg含量显著升高,诱发胆囊运动功能下降,胆囊胆汁淤积,是妊娠期胆囊结石形成的重要原因。

Effect of estrogen on the gallbladder motility in pregnant guinea pig

Objective To explore the effects of estrogen on the gallbladder motility and the role in the development of gallstone formation in pregnant guinea pig. Methods Sixty female guinea pigs were divided randomly into experimental group and control group. Animal models of pregnancy were established on experimental guinea pigs. The experimental group was divided into two groups:the 30 days of pregnancy and the 60 days of pregnancy. The serum estrogen(E2) and progesterone(Pg) level,also estrogen and progesterone receptors in the gallbladder were evaluated by using chemiluminescence and PAP immunohistochemical method in all animals. The gallbladder fasting volume(FV) and the gallbladder fasting bile volume (FB) were measured respectively. Results Gallstone formation were found in 3 guinea pigs of the 60th day in pregnancy. The levels of serum E2 and Pg,also the positive expression rates of ER and PR in gallbladder between the two groups were significantly different. So like was significantly about the FV and FB between the 60 days and 30 days of pregnancy and the control group(P< 0.001). Conclusion The increase of serum E2 and Pg level might play an important role in the down regulation gallbladder motility and gallstone formation in pregnancy.