TG、VLDL和OX-VLDL对L-02和HLF细胞增殖及合成细胞外基质的影响

目的探讨脂质对肝细胞和成纤维细胞生物学活性的影响。方法以ＭＴＴ法、透明质酸（ＨＡ）放免测定法及３Ｈ－脯氨酸掺入法，观察４种不同浓度的甘油三酯（ＴＧ）、极低密度脂蛋白（ＶＬＤＬ）和氧化极低密度脂蛋白（ＯＸ－ＶＬＤＬ）对无血清培养正常成人肝细胞（Ｌ－０２细胞）和人胚肺成纤维细胞（ＨＬＦ细胞）增殖及合成ＨＡ和胶原蛋白的影响。结果一定浓度的ＴＧ、ＶＬＤＬ和ＯＸ－ＶＬＤＬ可影响ＨＬＦ和（或）Ｌ－０２细胞的增殖，促进其胶原蛋白和ＨＡ的合成，其中以ＯＸ－ＶＬＤＬ的作用最为显著。结论肝内脂质，特别是ＯＸ－ＶＬＤＬ过多，可通过促进肝细胞和成纤维细胞合成细胞外基质诱导肝纤维化形成。

Effects of TG,VLDL,OX-VLDL on proliferation of L-02 and HLF cell lines and synthesis of extracellular matrix

Objective To explore the effects of lipid on biological behaviour of hepatocytes and fibroblasts. Methoda Effects of triglyceride(TG), very low density lipoprotein(VLDL) , and oxidized very low density lipoprotein(OX-VLDL) in four kinds of different concentration on proliferation of normal adult hepatocyte line(L-02) and human lung fibroblasts line (HLF) in aerua-free culture medium, and synthesis of collagenous protein and hyaluronic acid(HA) were observed by means of MTT measuring the incorporation of 3H-proline and radioimmunassy, respectively. Results TG,VLDL,OX-VLDL at a certain concentration can influence the prolifera- tion of L-02 and/or HLF cell lines, and promote the synthesis of collagen and HA, of which the effects of OX-VLDL is most prominent. Conclusion Excess of TG and its lipoprotein(OX-VLDL in particular) in the liver might induce hepatic fibrogenesis by promoting the synthesis of extracellular matrix by hepatocyte and fibroblasts.