精氨酸酶2在肝细胞癌组织中的表达及临床病理意义

目的 研究精氨酸酶2(Arg-2)在肝细胞癌(HCC)组织中的表达及临床病理意义.方法 收集113例HCC组织及癌旁肝组织,其中29例伴有癌旁异型增生结节(DN),另取12例正常肝组织.运用蛋白质印迹分析法检测Arg-2蛋白在15例HCC组织、癌旁肝组织和12例正常肝组织中的表达；运用免疫组织化学方法检测Arg-2在113例HCC、癌旁肝组织及DN中的表达情况,并分析其与HCC各临床病理特征间的关系以及鉴别诊断价值.结果 蛋白质印迹分析结果显示,Arg-2在正常肝组织和癌旁肝组织中均不表达,在HCC组织中表达升高(P＜0.01)；免疫组织化学结果显示,Arg-2阳性表达于肝癌细胞质内,阳性率为77.0％(87/113),其表达与HCC组织学分级相关(P＜0.05)；Arg-2在低级别DN中无表达,在高级别DN中阳性表达率为14.3％(2/14),而在高分化HCC中阳性表达率为66.7％(8/12),差异有统计学意义(P＜0.05).结论 Arg 2在HCC中高表达,并且与HCC组织学分级相关,其可能参与HCC的发生、发展过程.检测Arg-2表达有助于鉴别高分化HCC与DN.

Expression of arginase-2 in hepatocellular carcinoma tissues and its clinicopathological significance

Objective To investigate the expression of arginase-2 (Arg-2) in hepatocellular carcinoma (HCC) tissues and to discuss its clinicopathological significance. Methods Totally 113 HCC samples and corresponding adjacent liver tissues were collected. Among them 29 cases were characterized by the presence of dysplastic nodules (DN). Another 12 samples of normal liver tissues were collected as controls. Western blotting analysis was used to detect the Arg-2 protein expression in 15 HCC samples, corresponding adjacent liver tissues and 12 control samples. Immunohistochemical staining was performed to detect the Arg-2 expression in 113 HCC samples, corresponding adjacent liver tissues and DN samples. The correlation of Arg-2 expression with clinicopathological features of HCC was analyzed. Results Western blotting analysis revealed no Arg-2 expression in adjacent liver tissues and normal liver tissues, while the expression was significantly increased in HCC tissues (P<0.01). Immunohistochemical staining showed that Arg-2 was expressed in the cytoplasm of HCC cells, with a positive rate of 77.0% (87/113). Arg-2 expression was found to be correlated with the histological grades of HCC tissue (P<0.05). Arg-2 was not expressed in low-grade DN; its positive rate was 14.3% (2/14) in the high-grade DN and 66.7% (8/12) in well-differentiated HCC (P<0.05). Conclusion Arg-2 is highly expressed in the HCC tissues and its expression is associated with histological grades of HCC, and it may be involved in the development and progression of HCC. Detecting Arg-2 expression may help to differentiate HCC from DN.