| 竹灵消嫩茎再生系统建立研究 |
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| 引用本文： | 丛英子,顾元辉,刘润天,张冬艳,姜长阳.竹灵消嫩茎再生系统建立研究[J].辽宁中医学院学报,2010(11):71-73. |
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| 作者姓名： | 丛英子 顾元辉 刘润天 张冬艳 姜长阳 |
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| 作者单位： | 辽宁师范大学生命科学学院,辽宁大连116029 |
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| 基金项目： | 辽宁省高等教育教学改革研究项目（3-4）; 辽宁师范大学教学改革项目（LSJG：20090108） |
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| 摘 要： | 以竹灵消嫩茎为材料,进行了愈伤组织诱导和分化,试管苗的生根、移栽和移植的研究,建立起竹灵消嫩茎的再生体系。结果证明：MS＋BA0.5mg.L^-1＋24,-D 1.8mg.L^-1-2.0mg.L^-1是愈伤组织诱导的理想培养基;MS＋AgNO30.6mg.L^-1＋KT0.7mg.L^-1＋NAA0.1 mg.L^-1是愈伤组织分化培养的理想培养基;将不定芽下部切口在50mg.L^-1IAA无菌溶液中处理10min后接种到1/2MS0培养中进行生根培养的方法是生根培养的理想方法;移植到山坡上的试管苗长势旺盛,根系发达,当年开花结实。
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| 关 键 词： | 竹灵消 组织培养 再生体系 |
Research on Establishing the Regeneration System of Cynanchum Paniculatum |
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| CONG Ying-zi,GU Yuan-hui,LIU Run-tian,ZHANG Dong-yan,JIANG Chang-yang.Research on Establishing the Regeneration System of Cynanchum Paniculatum[J].Journal of Liaoning College of Traditional Chinese Medicine,2010(11):71-73. |
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| Authors: | CONG Ying-zi GU Yuan-hui LIU Run-tian ZHANG Dong-yan JIANG Chang-yang |
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| Institution: | (College of Life Science,Liaoning Normal University,Dalian 116029,Liaoning,China) |
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| Abstract: | The induction and differentiation of the callus were studied for young stem of Cynanchum inamoenum and then rooting and transplanting of tube plantlets were also studied,in the end an asexual system of Cynanchum inamoenum were successfully carried out in the experiment.The results showed that MS＋BA0.5mg.L^-1＋2,4-D 1.8mg.L^-1-2.0mg.L^-1 was the optimum medium for callus induction.The ideal medium for callus differentiation was MS＋AgNO30.6mg.L^-1＋KT0.7mg.L^-1＋NAA0.1mg.L^-1.The method of rooting was that transfer into the 1/2MS media after the cut of adventitious bud was treated in the 50mg.L^-1IAA for 10min.The seedling grew well with flourishing root and blooming and seeding in the former years. |
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| Keywords: | Cynanchum inamoenum tissue culture regeneration system |
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