Further evidence for preferential production of IgG2 anti-DNP antibody in guinea pigs immunized with DNP-Escherichia coli |
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| Affiliation: | 1. Department of Pharmaceutical Biosciences, Science for Life Laboratory, Uppsala University, Uppsala, Sweden;2. Immuneed AB, Uppsala, Sweden;3. Department of Immunohematology & Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands;4. Department of Immunology Genetics and Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden;5. Department of Bio-organic Synthesis, Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands;6. Institute for Translational Vaccinology Intravacc, Bilthoven, The Netherlands;3. Montreal Diabetes Research Center, Research Center of the University of Montreal Hospital Center (CRCHUM), and Department of Medicine, University of Montreal, Quebec H2X 0A9, Canada;4. Institut de Génomique Fonctionnelle, CNRS UMR 5203, INSERM U661, Universités de Montpellier 1 & 2, 34060 Montpellier, France;5. Department of Biochemistry and Molecular Medicine, University of Montreal, Quebec H3C 3J7, Canada;1. Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98102, USA;2. Molecular and Cellular Biology Graduate Program, University of Washington and Fred Hutchinson Cancer Research Center, Seattle, WA 98195, USA;3. Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98102, USA;4. Department of Medicine, University of Washington, Seattle, WA 98195, USA;5. Computational Biology Program, Fred Hutchinson Cancer Research Center, Seattle, WA 98102, USA |
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| Abstract: | As reported previously, radioimmunoelectrophoretic analysis of guinea pig antisera to 2,4-dinitrophenylated cells of Escherichia coli (DNP-E. coli) revealed that synthesis of antibodies to 2,4-dinitrophenyl residues (DNP) was restricted to one of the IgG antibodies, IgG2, when guinea pigs were immunized with the antigen in both the presence and absence of Freund's adjuvants. In order to confirm further this unique antibody response to DNP-E. coli, the subclass of anti-DNP antibodies produced was analyzed by DEAE-cellulose chromatography, electrofocusing and determination of antibody activities. The results so far obtained indicated that about 97% of the antibodies produced was of the IgG2 subclass and only a trace of the IgG1 antibody was produced, independently of the mode of immunization, though a small amount of IgG1 anti-DNP antibody was produced concomitantly on increasing the immunizing dose to 3.0 mg in saline. Furthermore, the cell-free extract prepared from DNP-E. coli also induced the preferential production of IgG2 anti-DNP antibody. |
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