灯盏花乙素的药动学研究

 目的建立大鼠血浆中灯盏花乙素的HPLC-ECD测定方法,研究大鼠静注及灌胃给药灯盏花乙素的药物动力学。方法色谱柱Kromasil C₁₈柱(4.6 mm×150 mm,5μm)；流动相pH2.6的50 mmol·L^-1磷酸盐缓冲液-甲醇-四氢呋喃(60：40：10)；流速1 mL·min^-1；分析电压100 mV。结果灌胃给药后,约在1.0 h,血药浓度达第一个高峰,约在5.0 h,血药浓度达第二个峰。结论灯盏花乙素静注给药的药-时曲线符合三室模型,经剂量校正,灌胃给药的绝对生物利用度为2.20%。

Study on Pharmacokinetics of Scutellarin in Rats

OBJECTIVE To develop a HPLC-ECD method for determining scutellarin in rat plasma and to study the pharmacokinetics of scutellarin in rats.METHODS The analytical column was Kromasil C₁₈(4.6mm×250mm,5um),and the mobile phase consisted of methanol, 50mmol·L^-1 phosphate buffer(adjusted by phosphoric acid to pH2.6),and tetrahydrofuran(40:60:10),the flow rat was 1.0mL·min^-1,the potential electrode voltage was 100 mV.RESULTS After oral administration of scutellarin to rats at the doses of 34 mg·kg^-1,the plasma concentration of scutellarin reached its first peak in 1.0h,with the second peak in about 5.0 h.The concentration-time curve of scutellarin and the main pharmacokinetic parameters were obtained.CONCLUSION The pharmacokinetic behavior of scutellarin iv belongs to the three-compartment model.The absolute bioavailability of scutellarin for oral administration was 2.20%.