抗独特型抗体对马来丝虫感染沙鼠的保护性免疫效果观察

目的 :研究抗丝虫抗独特型抗体 (抗 fil-抗 Id- Ab)对丝虫感染沙鼠的保护性免疫作用。方法 :从班氏丝虫病乳糜尿和鞘膜积液患者血清中分离含有高滴度的 Ig G,免疫家兔 ,获得兔抗 fil-抗 Id- Ig G,免疫沙鼠 ,再用马来丝虫感染期幼虫攻击 ,观察其免疫效果。结果 :抗 fil-抗 Id- Ig G一次脾内注射或多次皮下及腹腔内免疫后 ,50 %和 80 %沙鼠产生保护性免疫效果 ,沙鼠体内查不到微丝蚴和成虫 ,而从马来丝虫成虫可溶性抗原或兔抗正常人 Ig G为对照组的沙鼠中 ,80 %沙鼠感染丝虫 ,与正常感染组相近似。结论 :用抗 fil-抗 Id- Ab对马来丝虫感染沙鼠 ,证实有保护性免疫作用。

EFFECT OF ANTI-IDIOTYPIC ANTIBODY IMMUNIZATION ON PROTECTIVE IMMUNITY IN JIRDSAGAINST BRUGIA MALAYI

AIM:To assess the protective immunity engendered by anti- filaria- anti- idiotypic anti- body(anti- fil- anti- Id- Ab) in jirds(Meriones unguiculatus) against Brugia malayi.METH- ODS:Anti- fil- anti- Id- Ab was prepared from rabbits immunized with anti- fil- Ig G separated from high- titer sera from bancroftian filariasis patients with chyluria or hydrocele. Twenty two healthy jirds were randomly allocated to3groups.Group1 (7jirds) were each immu- nized with a single intrasplenic administration of anti- fil- anti- Id- Ig G(4jirds) or B.malayi soluble antigen (3jirds) . Group 2 (1 0 jirds) were each immunized with 3repeated sc and ip administration of anti- fil- anti- Id- Ig G(5 jirds) at7- 1 0 - day intervals or B.malayi soluble anti- gen(5 jirds) .Group3(5 jirds) served as challenge controls.Ten days after the last immu- nization,each jird in all three groups were challenged by ip route with 2 0 0 B.malayi third stage larvae.RESUL TS:Jirds immunized with a single administration or3repeated adminis- tration of anti- fil- anti- Id- Ig G could induce5 0 % and80 % protective immunity,respectively. No microfilaria or adult worms could be found in these jirds. In control group using rabbit anti- human Ig G or B.malayi adult worm soluble antigen,no apparent protective immunity was found.CONCL USION:Immunization of jirds with anti- fil- anti- Id- Ig G resulted in pro- tected immunity (5 0 % - 80 % ) against B.malayi third stage larvae infection.