Behavioral and sensory changes after direct ischemia-reperfusion injury in rats. |
| |
Authors: | Natalia Gorodetskaya Jörn Schattschneider Wilfrid Jänig Ralf Baron |
| |
Affiliation: | 1. Division of Experimental Cardiology, Department of Cardiology, Thoraxcenter, Cardiovascular Research Institute COEUR, Erasmus MC, University Medical School, Rotterdam, The Netherlands;2. Department of Pathophysiology and Gerontology, Medical School, University of Pécs, Hungary;3. Department of Physiology, New York Medical College, Valhalla, NY, USA;4. Division of Cardiovascular Medicine, University at Buffalo and the Western New York Department of Veterans Affairs Health System, Buffalo, NY, USA;1. Department of Pharmacology, Dalhousie University, PO Box 15000, 5850 College St, Halifax, NS B3H 4R2, Canada;2. Department of Medicine (Geriatric Medicine), Dalhousie University, PO Box 15000, 5850 College St, Halifax, NS B3H 4R2, Canada;3. Institute of Cardiovascular Sciences, University of Manchester, Manchester, UK |
| |
Abstract: | Complex regional pain syndromes (CRPS) are disabling pain syndromes that can develop after trauma or minor tissue injury affecting a limb. Characteristics of CRPS are sensory signs and symptoms, autonomic abnormalities, trophic changes and an impaired motor function. Pathophysiological mechanisms for the development of CRPS are still a matter of investigation. Based on clinical data and investigations of CRPS patients it is hypothesized that tissue hypoxia and inflammation are important for the development of CRPS. The aim of the current study was therefore to examine if direct ischemia-reperfusion injury can induce behavior in rats with symptoms present in patients with CRPS. After baseline behavior measurements the femoral artery of Wistar rats was ligated for 3h with consecutive reperfusion. Sham-operated rats underwent the same preparation except ligation of the artery. Subsequent behavioral testing (observations of spontaneous pain behavior, paw withdrawal to mechanical, noxious mechanical, cold and heat stimuli) was performed up to two months after surgery. Both in rats that underwent ischemia and in sham-operated rats no obvious changes of hindpaw tissue were observed after ischemia-reperfusion injury (trophic changes, edema, differences in skin color or temperature). In behavioral tests only minor changes were observed, these being not different between postischemic rats and sham-operated rats. Using Wistar rats, our data do not support the idea that an ischemia-reperfusion injury can play a major role in the development of CRPS. |
| |
Keywords: | CRPS Ischemia‐reperfusion‐injury Inflammation Post‐ischemia pain Rat |
本文献已被 ScienceDirect 等数据库收录! |
|