西藏地区60例慢性乙型肝炎患者病毒基因分型及分布特点

目的 了解西藏地区HBV基因型分型及分布特点.方法 选择2000年1月至2004年3月西藏地区HBsAg阳性的藏族HBV感染者60例.采用S基因序列分析法检测HBV基因型,并分析相应的前C/C区基因序列特点.结果 60例HBV样本基因型中,59例为Dc混合型,1例为Dbc混合型,其前C/C基因与B、C基因型均发生重组.结论 本次调查HBV基因型为Dc混合型和Dbc混合型,无纯D型及其他基因型.

Genotypic characteristics of hepatitis B virus isolated from 60 Tibet patients

Objective To investigate the genotypic characteristics of hepatitis B virus(HBV) in Tibet.Methods Serum samples from 60 cases of hepatitis B in Tibet Autonomous Region were collected from January 2000 to March 2004.HBV genotypes were analyzed by sequencing S region and precore/core region.Results HBV isolated from 60 cases were all found to be D genotype by S region sequencing.Dc mixture was found in 59 cases,showing the recombination between their precore/core gene and genotype B virus at 1804-2299 nucleotide.The other one case showed Dbc mixture genotype,showing recombination between its precore/core gene and genotype B and C genes.Conclusions All Tibet cases in this study show mixture genotype D with recombination with genotvpe C or both genotype B and C at precore/core region.No case of pure genotype D is found.