| 白花蛇舌草水提取物对多药耐药白血病细胞HL-60/ADR的作用 |
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| 引用本文： | 赵诗云,尹小明,吴东风,陈厚爱,孙敬,曾黎峰,熊美丽,伍柏青,龚旭疆,申细保. 白花蛇舌草水提取物对多药耐药白血病细胞HL-60/ADR的作用[J]. 四川中医, 2010, 0(2): 55-57 |
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| 作者姓名： | 赵诗云 尹小明 吴东风 陈厚爱 孙敬 曾黎峰 熊美丽 伍柏青 龚旭疆 申细保 |
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| 作者单位： | 江西省中医药研究院;江西省人民医院; |
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| 摘 要： | 目的:研究白花蛇舌草水提取物对多药耐药白血病细胞HL-60/ADR的抑制作用及机制。方法:采用MTT比色试验观察白花蛇舌草水提取物对多药耐药细胞HL-60/ADR的抑制作用。采用光镜、电镜技术观察细胞形态结构的改变,利用流式细胞术(FCM)检测细胞凋亡率。结果:①白花蛇舌草水提取物能明显抑制HL-60/ADR细胞的生长。②形态学改变,呈典型的凋亡特征。③流式细胞术证实,凋亡率也呈时间和剂量的依赖性。结论:白花蛇舌草水提取物对多药耐药细胞HL-60/ADR的生长具有极强的抑制作用,诱导其凋亡是其主要机制。
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| 关 键 词： | 白花蛇舌草水提取物 多药耐药 HL-60／ADR 凋亡 |
Study the Inhibitory Action and Inducing Apoptosis of Aqueous Extract from Hedyotic Diffusa on Multidrug Resistance Leukemic Cell Line HL-60/ADR |
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| Zhao Shiyun Yin Xiaoming Wu Dongfeng Cheng Houai Sun Jing Zeng Lifeng Xiong Meili Wu Boqing Gong Xujiang Sheng Xibao .Jiangxi Provincial Traditional Chinese Medicine Academy.Jiangxi Provincial People's Hospital. Study the Inhibitory Action and Inducing Apoptosis of Aqueous Extract from Hedyotic Diffusa on Multidrug Resistance Leukemic Cell Line HL-60/ADR[J]. Journal of Sichuan of Traditional Medicine, 2010, 0(2): 55-57 |
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| Authors: | Zhao Shiyun Yin Xiaoming Wu Dongfeng Cheng Houai Sun Jing Zeng Lifeng Xiong Meili Wu Boqing Gong Xujiang Sheng Xibao .Jiangxi Provincial Traditional Chinese Medicine Academy.Jiangxi Provincial People's Hospital |
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| Affiliation: | Zhao Shiyun Yin Xiaoming Wu Dongfeng Cheng Houai Sun Jing Zeng Lifeng Xiong Meili Wu Boqing Gong Xujiang Sheng Xibao 1.Jiangxi Provincial Traditional Chinese Medicine Academy(Nangchang Jiangxi 330077,China)2.Jiangxi Provincial People's Hospital(Nangchang Jiangxi 330006,China) |
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| Abstract: | Objectives ： To study the inhibitory action and mechanism of aqueous extract from Hedyotic Diffusa on multidrug resistance leukemic cell line HL - 60/ADR. Methods ： To observe the depressant effect of aqueous extract from Hedyotic Diffusa on muhidrug structure by light microscope and electron microscope, to detect the apoptosis rate by Flow Cytometry （ FCM ） Results： （1）Aqueous extract from Hedyotic Diffusa could effectively inhibit the proliferation of HL -60/ADR ceils. （2）The morphological changes are the typical character of apoptosis. （3）The apoptotic rate has dependence of dosage and time have been confirmed by Flow Cytometry （ FCM ） . Conclusions ： Aqueous extracts from Hedyotic Diffusa could effectively inhibit the proliferation of multidrug resistance leukemic cell line HL - 60/ADR, induce HL - 60/ADR to apoptosis is one of its mechanism. |
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| Keywords: | Aqueous extract from Hedyotic Diffusa multidrug resistance HL-60/ADR apoptosis |
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