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| 肺癌细胞株E - cadherin的表达状态及其与启动子甲基化的关系 | |
| 引用本文： | 郝立宏,杨晓燕,宫琳琳,赵瑾瑶,宋 阳,杜 莎,王 兰,刘 鹭,熊 海. 肺癌细胞株E - cadherin的表达状态及其与启动子甲基化的关系[J]. 大连医科大学学报, 2011, 33(4): 325-329 |
| 作者姓名： | 郝立宏 杨晓燕 宫琳琳 赵瑾瑶 宋 阳 杜 莎 王 兰 刘 鹭 熊 海 |
| 作者单位： | 1. 大连医科大学蛋白质组学实验室,辽宁大连,116044 2. 大连医科大学蛋白质组学实验室,辽宁大连116044;大连医科大学附属第二医院肿瘤科,辽宁大连116027 3. 大连医科大学附属第一医院胸外科,辽宁大连,116001 |
| 摘 要： | [目的]研究肺癌细胞株上皮钙粘蛋白(E - cadherin)的表达与基因启动子甲基化状态的关系.[方法]采用免疫细胞化学法检测肺癌细胞株NCI - H460、SPCA和A549的E- cadherin蛋白表达；半巢式甲基化特异性PCR法(MSP)和微流控芯片技术检测E- cadherin基因启动子甲基化状态.[结果]... |
| 关 键 词： | 上皮钙粘蛋白 启动子甲基化 MSP 微流控芯片技术 |
Protein expression and promoter methylation status of E-cadherin in lung cancer cell lines |
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| HAO Li-hong,YANG Xiao-yan,GONG Lin-lin,ZHAO Jin-yao,SONG Yang,DU Sh,WANG Lan,LIU Lu and XIONG Hai. Protein expression and promoter methylation status of E-cadherin in lung cancer cell lines[J]. Journal of Dalian Medical University, 2011, 33(4): 325-329 | |
| Authors: | HAO Li-hong YANG Xiao-yan GONG Lin-lin ZHAO Jin-yao SONG Yang DU Sh WANG Lan LIU Lu XIONG Hai |
| Affiliation: | 1,3(1.Department of Histology and Embryology,Dalian Medical University,Dalian 116044,China;2.Department of Oncology,the Second Affiliated Hospital of Dalian Medical University,Dalian 116027,China;3.Department of Thoracic Surgery,the First Affiliated Hospital of Dalian Medical University,Dalian 116011,China) |
| Abstract: | [Objective] To investigate the relationship between the promoter methylation status and protein expression of E-cadherin in lung cancer cell lines.[Methods] The protein expressions of E-cadherin in lung cancer cell lines NCI-H460,SPCA and A549 were examined by immunocytochemistry.The promoter methylation status of E-cadherin was detected by methylation-specific PCR(MSP) and microfluidics.[Results] The weak expressions of E-cadherin in NCI-H460,SPCA and A549 were detected,the weakest in A549.The promoter methylation of E-cadherin occurred in all three cell lines and the highest percentage of methylation in A549,which was in agreement with the protein expression.[Conclusions] The promoter methylation can be a cause of E-cadherin its down-regulation in lung cancer cell lines. |
| Keywords: | E-cadherin methylation MSP microfluidics |
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