NF - κB抑制剂PDTC对小鼠肝癌细胞生长的影响

目的]观察核因子-κB(NF- κB)抑制剂吡咯烷二硫代氨基甲酸盐(PDTC)对小鼠肝癌Hca -F细胞生长的影响.方法]用含PDTC的完全培养基体外培养Hca -F细胞,用MTT法测Hca -F细胞增殖抑制率.体内实验中所有小鼠均接种Hca -F并随机分为两组:攻击对照组腹腔注射生理盐水,PDTC治疗组腹腔注射P...

Role of PDTC in regulating the growth of mouse Hca-F cell

Objective] To observe the effects of pyrro1idine dithiocarbamate(PDTC),an inhibitor of NF—κB,on the growth of mouse Hca-F cell in vitro and in vivo.Methods] Hca-F cells were cultured in complete medium containing PDTC in vitro.MTT assay was used to detect the inhibition of growth of Hca-F cell.In vivo,the mice were inoculated with Hca-F cells and divided randomly into two groups: mice of challenge control group were injected with normal saline,while those of PDTC treatment group were injected with PDTC solution.The volume of tumor and body weight of mice were measured every day.Mice were killed on day 10 after treatment.Malondialchehyche(MDA) content of tumor tissue was assayed with MDA reagent kit and TNF-content was assayed with RIA.Results] PDTC inhibited the proliferation of Hca-F cells in vitro,the proliferation inhibition rate was 85.4% on concentration 25 μg/mL.In vivo,differences in the volume,weight of tumor,content of MDA and TNF-α level of tumor tissue supernant between the two groups were not significant(P>0.05).Conclusion] PDTC can inhibit the proliferation of Hca-F cell in vitro.