| 海藻糖高产株Brevibacterium sp SY361海藻糖磷酸化酶(EC 2.4.1.64)的纯化 |
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| 引用本文： | 王蕾,黄瑞,顾冠彬,万海燕. 海藻糖高产株Brevibacterium sp SY361海藻糖磷酸化酶(EC 2.4.1.64)的纯化[J]. 苏州大学学报(自然科学版), 2007, 27(1): 69-71 |
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| 作者姓名： | 王蕾 黄瑞 顾冠彬 万海燕 |
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| 作者单位： | 苏州大学医学院微生物学教研室 江苏苏州215123 |
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| 摘 要： | 目的研究分离纯化海藻糖高产株Brevibacterium sp SY361中海藻糖磷酸化酶的工艺,获得高纯度的海藻糖磷酸化酶。方法大量培养细菌Brevibacterium sp SY361,超声粉碎细胞,通过硫酸铵分级沉淀、DEAE-SephadexA-25柱层析和Sephadex G-200凝胶过滤等步骤纯化其海藻糖磷酸化酶,并通过SDS-PAGE检测纯化效果。结果经纯化后的海藻糖磷酸化酶纯度提高了35.6倍,比活力达到7.48U/mg,SDS-PAGE呈1条带。结论纯化后的海藻糖磷酸化酶已达到纯度要求,可用于酶性质的研究。
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| 关 键 词： | 海藻糖磷酸化酶 纯化 |
| 文章编号： | 1673-0399（2007）01-0069-03 |
| 修稿时间： | 2006-02-24 |
Study on the Purification of Trehalose Phosphorylase from a Rich Trehalose-producing Strain Brevibacterium sp SY361 |
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| WANG Lei,HUANG Rui,GU Guan-bin,WAN Hai-yan. Study on the Purification of Trehalose Phosphorylase from a Rich Trehalose-producing Strain Brevibacterium sp SY361[J]. Suzhou University Journal of Medical Science, 2007, 27(1): 69-71 |
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| Authors: | WANG Lei HUANG Rui GU Guan-bin WAN Hai-yan |
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| Affiliation: | Dept of Microbiology , Medical School of Suzhou University, Jiangsu Suzhou 215123, China |
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| Abstract: | Objective To study the process of extracting and purifying the trehalose phosphorylase from a rich trehalose-producing strain Brevibacterium sp SY361.Methods The partial purified trehalose phosphorylase was prepared from a cell-free extract of Brevibacterium sp SY361 by precipitation with(NH4)2SO4,DEAE-Sephadex A-25 chromatography and Sephadex G-200 gel filtration successively.SDS-PAGE was applied to check the purity of the enzyme.Results The enzyme was purified about 35.6 fold from the cell-free extract,and the specific activity reached 7.48 U/mg.The enzyme showed a single band on SDS-PAGE.Conclusion The purified trehalose phosphorylase meets the requirements of the further research. |
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| Keywords: | Brevibacterium sp SY361 |
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