| 乙肝病毒外膜大蛋白检测的临床意义 |
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| 引用本文： | 马洪滨,孙志强,史佳彬,李筱涵,徐军,郭静霞,杨丽华,王雪飞,李伯安,毛远丽. 乙肝病毒外膜大蛋白检测的临床意义[J]. 军事医学科学院院刊, 2007, 31(3): 262-263 |
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| 作者姓名： | 马洪滨 孙志强 史佳彬 李筱涵 徐军 郭静霞 杨丽华 王雪飞 李伯安 毛远丽 |
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| 作者单位： | 解放军第302医院临床检验中心,北京,100039 |
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| 摘 要： | 目的:探讨乙肝病毒外膜大蛋白(HBV-LP)检测的临床意义.方法:随机选取203例乙肝患者血清,采用酶联免疫吸附试验(ELISA)检测HBV-LP、Pre-S1蛋白及HBV M,实时定量PCR方法检测HBV DNA.结果:LP的检出阳性率与HBV DNA的检出阳性率相关性具有统计学意义,且HBV DNA 拷贝数的对数值与HBV-LP 表达具有相关关系(r＝0.902);LP检出阳性率与Pre-S1蛋白检出阳性率差异具有统计学意义;不同HBV M模式检测LP检出阳性率差异不具有统计学意义,但26例HBeAg阴性患者血清LP检测为阳性.结论:血清中HBV-LP的含量与HBV DNA的拷贝数具有较好的相关性;血清中HBV-LP的检测是对HBV M检测的补充.
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| 关 键 词： | 肝炎病毒,乙型 病毒包膜蛋白质类 DNA,病毒 聚合酶链反应 乙肝 病毒 外膜 蛋白检测 临床 统计学意义 detection protein large hepatitis B virus 补充 率相关性 含量 患者血清 阴性 HBeAg 模式 差异 关系 表达 |
| 文章编号： | 1000-5501（2007）03-0262-03 |
| 修稿时间： | 2007-03-01 |
Significance of hepatitis B virus large protein detection |
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| MA Hong-Bin,SUN Zhi-Qiang,SHI Jia-Bin,LI Xiao-Han,XU Jun,GUO Jing-Xia,YANG Li-Hua,WANG Xue-Fei,LI Bo-An,MAO Yuan-Li. Significance of hepatitis B virus large protein detection[J]. Bulletin of the Academy of Military Medical Sciences, 2007, 31(3): 262-263 |
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| Authors: | MA Hong-Bin SUN Zhi-Qiang SHI Jia-Bin LI Xiao-Han XU Jun GUO Jing-Xia YANG Li-Hua WANG Xue-Fei LI Bo-An MAO Yuan-Li |
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| Affiliation: | Center of Clinical Laboratory,No, 302 Hospital of PLA, Beijing 100039, China |
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| Abstract: | Objective: To explore the significance of hepatitis B virus large protein(LP) detection.Methods: PreSl-Ag,LP and HBV markers in sera were measured by enzyme linked immunosorbent assay(ELISA) in 203 cases of hepatitis B.Serum HBV DNA level was quantitatively detected by real-time polymerase chain reaction (PCR).Results: The correlation between positive rates of LP and HBV DNA had statistical significance;HBV-LP expression was markedly correlated with the logarithm of HBV DNA level(r=0.902).The difference of positive rates between LP and the PreSl-Ag had statistical significance.The expression of HBV-LP was not significantly different among various patterns of HBV serological markers.Conclusion: There is good correlation between the copies of HBV DNA and the serum level of HBV-LP.Serum LP detection is a complementary means for traditional HBV M. |
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| Keywords: | hepatitis B virus viral envelope proteins DNA viral polymerase chain reaction |
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