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| 人格拉利素新抗菌肽的抗菌作用研究 | |
| 引用本文： | 尹丽莉,杨超文,杨瑞馥,端青,何君,赵光,沙继斌,胡锴勋,赵士富.人格拉利素新抗菌肽的抗菌作用研究[J].军事医学科学院院刊,2007,31(4):349-351. |
| 作者姓名： | 尹丽莉 杨超文 杨瑞馥 端青 何君 赵光 沙继斌 胡锴勋 赵士富 |
| 作者单位： | 1. 军事医学科学院放射与辐射医学研究所,北京,100850 2. 军事医学科学院微生物流行病研究所,北京,100071 3. 西安交通大学生命科学与技术学院,西安,710049 4. 军事医学科学院附属医院,北京,100071 |
| 基金项目： | 国家重点基础研究发展计划(973计划) |
| 摘 要： | 目的:进一步证实人格拉利素(hGlyrichin)抗菌肽的抗菌功能.方法:根据人格拉利素的氨基酸序列,设计并合成了19个氨基酸长度的多肽(pCM-19),采用试管法和菌落生成法分析该肽对靶细菌生长的影响.结果与结论:pCM-19溶液与不同靶细菌共孵育后,明显抑制了细菌的生长,对实验室常用的BL-21工程菌和多种致病菌(包括杆菌、球菌)的生长都有抑制作用.更为有趣的是对耐甲氧西林金黄色葡萄球菌的生长有明显抑制作用,显示了其在抗耐药菌感染中的良好前景. |
| 关 键 词： | 人格拉利素 pCM-19 抗菌肽 抗菌药 氨基酸序列 人格 抗菌肽 抗菌作用 研究 based peptide antibacterial novel 前景 耐药菌感染 显示 黄色葡萄球菌 耐甲氧西林 抑制作用 杆菌 致病菌 工程菌 实验室 细菌生长 共孵育 |
| 文章编号： | 1000-5501（2007）04-0349-03 |
| 修稿时间： | 2007-02-28 |
Antibacterial activity of a novel antibacterial peptide based on hGlyrichin |
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| YIN Li-Li,YANG Chao-Wen,YANG Rui-Fu,DUAN Qing,HE Jun,ZHAO Guang,SHA Ji-Bin,HU Kai-Xun,ZHAO Shi-Fu.Antibacterial activity of a novel antibacterial peptide based on hGlyrichin[J].Bulletin of the Academy of Military Medical Sciences,2007,31(4):349-351. | |
| Authors: | YIN Li-Li YANG Chao-Wen YANG Rui-Fu DUAN Qing HE Jun ZHAO Guang SHA Ji-Bin HU Kai-Xun ZHAO Shi-Fu |
| Abstract: | Objective:To further demonstrate the antibacterial activity of a novel hGlyrichin protein based on our previous experimental results.Methods:A peptide (pCM-19) was designed and synthesized according to the sequence of hGlyrichin after bioinformatic analysis. The growth status of target bacteria after incubation with pCM-19 was evaluated by means of monitoring the growth rate in the liquid culture and counting the number of colonies.Results and Conclusion:The growth of target bacteria was markedly inhibited after incubation with pCM-19. pCM-19 Has a broad spectrum of inhibitory activity against both pathogenic and non-pathogenic bacterial strains. Moreover, pCM-19 could also inhibit the growth of antibiotics-resistant bacterial strains,and it implies that pCM-19 could be used as a therapeutic agent to treat patients with infectious diseases. |
| Keywords: | hGlyrichin pCM-19 antimicrobial peptide anti-bacterial agent amino acid sequence |
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