3D Bioprinting of Biosynthetic Nanocellulose-Filled GelMA Inks Highly Reliable for Soft Tissue-Oriented Constructs |
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| Authors: | Alexandra I Cernencu Adriana Lungu Diana M Dragusin Izabela C Stancu Sorina Dinescu Liliana R Balahura Paul Mereuta Marieta Costache Horia Iovu |
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| Institution: | 1.Advanced Polymer Materials Group, University Politehnica of Bucharest, 011061 Bucharest, Romania; (A.I.C.); (D.M.D.); (I.C.S.); (H.I.);2.Department of Biochemistry and Molecular Biology, University of Bucharest, 050095 Bucharest, Romania; (S.D.); (L.R.B.); (M.C.);3.Horia Hulubei—National Institute for Physics and Nuclear Engineering (IFIN-HH), 30 Reactorului Street, 077125 Magurele, Romania;4.Academy of Romanian Scientists, 54 Splaiul Independentei, 050094 Bucharest, Romania |
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| Abstract: | Bioink-formulations based on gelatin methacrylate combined with oxidized cellulose nanofibrils are employed in the present study. The parallel investigation of the printing performance, morphological, swelling, and biological properties of the newly developed hydrogels was performed, with inks prepared using methacrylamide-modified gelatins of fish or bovine origin. Scaffolds with versatile and well-defined internal structure and high shape fidelity were successfully printed due to the high viscosity and shear-thinning behavior of formulated inks and then photo-crosslinked. The biocompatibility of 3D-scaffolds was surveyed using human adipose stem cells (hASCs) and high viability and proliferation rates were obtained when in contact with the biomaterial. Furthermore, bioprinting tests were performed with hASCs embedded in the developed formulations. The results demonstrated that the designed inks are a versatile toolkit for 3D bioprinting and further show the benefits of using fish-derived gelatin for biofabrication. |
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| Keywords: | 3D bioprinting methacrylamide-modified gelatin cellulose nanofibrils biomaterial inks 3D nanocomposite scaffolds |
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