溶血磷脂酸协同TGFβ1促进结肠癌细胞上皮间质转化的研究

目的探讨溶血磷脂酸(LPA)协同转化生长因子1(TGFβ1)的促上皮间质转化(EMT)作用。 方法观察TGFβ1及LPA诱导SW480细胞后细胞形态变化，Western blotting方法检测E-Cadherin、Vimentin的表达变化以及细胞增殖活性变化。 结果TGFβ1及LPA均能使SW480细胞形态发生EMT改变；两者联合诱导细胞EMT改变更为明显。空白对照组、LPA组、TGFβ1组、LPA组＋TGFβ1组，E-Cadherin表达依次下降；Vimentin表达未见明显差异。 结论LPA可能协同TGF-β1产生EMT作用，下调LPA的表达或可抑制肿瘤侵袭转移。

The effect and mechanism of lysophosphatidic acid oil metastasis and anti-apoptosis in colorectal carcinoma

ObjectiveThis study aimed to investigate whether LPA could synergize TGFβ1 to increase epithelial-to-mesenchymal transition(EMT). MethodsWestern blotting was used to examine the EMT markers: E-cadherin and Vimentin.MTT was used to examine the cell proliferation of 5-Fu and LOHP with or without LPA.Western blotting was used to examine the apoptosis markers Bcl-2, Bax and Caspese-3 after LPA treatment. ResultsTGFβ1 and LPA induced the morphology change of colorectal cancer cell line SW480, and combination group LPA/TGFβ1 induced more significant change.Compared with the LPA group, the difference of E-cadherin in blank control group 1%FBS, and TGFβ1 treatment group, the LPA/TGFβ1 co-treatment group were significant, while the difference of Vimentin was not significant. ConclusionLPA synergizes with TGF-β to play an important role in EMT.Inhibition of LPA may decrease the invasion and migration of colon cancer cells.