P21激活激酶1在甲状腺乳头状癌中的表达及临床意义的研究

目的探讨甲状腺乳头状癌(PTC)中P21激活激酶1(PAK1)的表达情况及其临床意义。 方法使用免疫组化法检测55例PTC和25例非恶性甲状腺组织中PAK1蛋白的表达情况,分析其与PTC临床病理参数的关系。 结果43.6% (24/55)的PTC组织中PAK1蛋白高表达或过表达,显著高于甲状腺良性组织的8.0%(2/25)(χ²=9.950,P=0.002)。在有腺体外浸润、临床分期较高、有淋巴结转移的PTC中PAK1蛋白的表达较高,差异均有统计学意义(χ²=5.832,P=0.016;χ²=4.448,P=0.035;χ²=4.990,P=0.025);而在不同年龄、性别、肿瘤大小、是否多发、是否为双侧等分组中差异均无统计学意义。 结论PAK1在PTC中呈高表达,可能参与肿瘤增殖、浸润和转移。

Expression and clinical significance of PAK1 in papillary thyroid carcinoma

ObjectiveTo investigate the expression of P21 activated kinase 1 (PAK1) protein in papillary thyroid carcinoma (PTC), and to figure out its clinical significance. MethodsWe used strept avidin-biotin complex (SABC), the sensitive immunohistochemistry, to detect the expression of PAK1 in eighty paraffin-embedded specimens, including 55 PTC and 25 benign thyroid nodules, and to explore its relationship with clinicopathological characteristics of PTC. Results43.6% of PTC specimens showed significantly higher expression of PAK1 protein than that of benign lesions of thyroid, which had only 8.0% positive expression rate (χ²=9.950, P=0.002). The expression of PAK1 was positively and significantly correlated with tumor invasion, advanced stage and lymph node metastasis (χ²=5.832, P=0.016; χ²=4.448, P=0.035; χ²=4.990, P=0.025), but it had no correlation with age, gender, tumor size, multiple tumors and bilateral tumors in PTC. ConclusionThe high expression of PAK1 in PTC is related to the possibility of proliferation, invasion and lymph node metastasis.