福建省临床分离非结核分枝杆菌菌种分布研究

目的 分析我院临床分离非结核分枝杆菌(NTM)菌株,探讨福建省NTM临床分离率和菌种分布情况。方法 对我院2009-2012年临床分离6 362株分枝杆菌进行结核分枝杆菌复合群(MTBC)与NTM鉴别,对鉴定为NTM菌株应用hsp65-和rpoB- PCR-RFLP进行菌种鉴定。结果 6 362株临床分离分枝杆菌共鉴别出649株NTM。鉴别出的649株NTM菌株,经hsp65-和rpoB-PCR-RFLP鉴定,菌种分布达24个种,其中菌株数量前3位菌种为:胞内分枝杆菌(M.intracellulare)302株(46.5%),鸟分枝杆菌(M. avium)138株(21.3%),脓肿分枝杆菌(M. abscessus)81株(12.5%)。结论 2009-2012 年福建省NTM临床分离率占分枝杆菌培养阳性菌株的10.2%,NTM种类多,以鸟-胞内分枝杆菌复合群(MAC)为福建省NTM的主要流行菌种,占总数的67.8%。

Distribution of nontuberculous mycobacteriaof clinical mycobacterium isolates from Fujian Province,China

To understand the species distribution of nontuberculous mycobacteria (NTM) in Fujian Province of China, we collected clinical Mycobacterium isolates in the Fuzhou Pulmonary Hospital from 2009 to 2012. A total of 6 362 clinical Mycobacteria isolates were identified as 5 713 (89.8%) M. tuberculosis complex and 649 (10.2%) NTM strains by conventional identification method. Then, by means of hsp65- and rpoB-PCR-RFLP methods, 649 NTM strains were identified as 24 species or complex of NTM, in which the top three species or complex with the highest occurrence frequency were M. intracellular, M. avium and M. abscessus, accounting for 48.5%, 21.3% and 12.5% respectively. The prevalence rate of NTM was 10.2% among Mycobacterium culture-positive patients. There are lots of NTM species infecting human being, and the most prevalence NTM species was M. avium complex accounting for 67.8% in Fujian Province.