系统性红斑狼疮患者CD4+ T细胞中miR-17和miR-20a 的表达及与病情活动的相关性研究

【摘要】 目的 探讨miR-17和miR-20a在系统性红斑狼疮（SLE）患者CD4+ T细胞中的表达及与疾病活动的相关性。 方法 应用实时荧光定量聚合酶链反应（PCR）检测SLE患者活动组17例、缓解组13例及健康对照组18例外周血CD4+ T细胞中miR-17和miR-20a的表达水平，并与SLE患者临床指标进行相关性分析。 结果 SLE患者组30例miR-17的表达水平（0.24 ± 0.08）显著高于健康对照组（0.15 ± 0.06），两组比较，P < 0.05；两组miR-20a的表达水平分别为0.19 ± 0.10和0.12 ± 0.09，两组比较，P < 0.05。SLE活动组、缓解组与健康对照组miR-17表达水平分别为0.27 ± 0.07、0.20 ± 0.05、0.16 ± 0.08，活动组高于缓解组（P < 0.05）和健康对照组（P < 0.05）；miR-20a表达水平三组分别为0.22 ± 0.10、0.15 ± 0.07、0.13 ± 0.09，活动组高于缓解组（P < 0.05）和健康对照组（P < 0.05）；缓解组miR-17和miR-20a的表达水平与健康对照组比较差异均无统计学意义（P > 0.05）。SLE患者组miR-17、miR-20a的表达与SLE活动指数（SLEDAI）均呈正相关（r = 0.45，P < 0.05和r = 0.38，P < 0.05），与抗dsDNA抗体滴度呈正相关（r = 0.54，P < 0.01和r = 0.46，P < 0.01），而与C3水平呈负相关（r = -0.43，P < 0.05和r = -0.42，P < 0.05）。 结论 SLE患者外周血CD4+ T细胞中miR-17、miR-20a表达上调，且miR-17、miR-20a表达水平与SLE病情活动相关。

Expressions of miR-17 and miR-20a in CD4+ T cells from patients with systemic lupus erythematosus and their correlations with disease activity

Wen Yunshi, Qin Haihong, Xu Jinhua. Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China Corresponding author: Xu Jinhua, Email: hsyyxjh@163.com 【Abstract】 Objective To investigate the expressions of miR-17 and miR-20a in CD4+ T cells from patients with systemic lupus erythematosus （SLE） and their correlations with disease activity. Methods CD4+ T cells were isolated from the venous blood of 30 patients with SLE and 18 healthy human controls. RNA was extracted from the CD4+ T cells, and real-time fluorescence-based quantitative PCR （qPCR） was performed to determine the expression levels of miR-17 and miR-20a. The intergroup comparison of miR-17 and miR-20a expression levels was done using t test and Mann-Whitney test, and the correlations of miR-17 and miR-20a expressions with clinical parameters were assessed using Pearson or Spearman correlation coefficients. Results The patients with SLE showed increased expression levels （2－△Ct） of miR-17 and miR-20a compared with the healthy controls （0.24 ± 0.08 vs. 0.15 ± 0.06 for miR-17, 0.19 ± 0.10 vs. 0.12 ± 0.09 for miR-20a, both P < 0.05）. The miR-17 and miR-20a expression levels were also significantly higher in patients with active SLE than in those with inactive SLE and the healthy controls （0.27 ± 0.07 vs. 0.20 ± 0.05 and 0.16 ± 0.08 for miR-17, 0.22 ± 0.10 vs. 0.15 ± 0.07 and 0.13 ± 0.09 for miR-20a, all P < 0.05）, but similar between the patients with inactive SLE and healthy controls （both P > 0.05）. The expression levels of both miR-17 and miR-20a were positively correlated with SLE disease activity index （SLEDAI） （r = 0.45, 0.38, respectively, both P < 0.05） and anti-dsDNA antibody level （r = 0.54, 0.46, respectively, both P < 0.05）, but negatively correlated with serum C3 levels （r = －0.43, －0.42, respectively, both P < 0.05）. Conclusions The expressions of miR-17 and miR-20a are increased in CD4+ T cells from patients with SLE, and correlated with the disease activity in SLE.