CD4+CD29+辅助性T细胞在脊髓损伤患者的表达与临床意义

目的探索CD4+CD29+辅助性T细胞在脊髓损伤患者的表达与临床意义。方法收集脊髓损伤患者75例, 以50例健康人作为对照组, 采用流式细胞术检测外周血CD4+CD29+辅助性T细胞及其胞内细胞因子γ干扰素(IFN-γ)及白介素4(IL-4)含量; 分别在伤后第3及7天复查三者, 并分析三者与Frankel分级的相关性。结果与正常人比较, 脊髓损伤患者CD4+CD29+T细胞及IL-4含量明显升高, 而IFN-γ含量明显降低(P < 0.05);治疗第3天, CD4+CD29+T细胞及IL-4较受伤第1天均有不同程度下降, 而IFN-γ不同程度回升, 其中以D及E级的改变明显(P < 0.05)。治疗第7天, CD4+CD29+T细胞及IL-4继续下降, IFN-γ继续回升, 其中D及E级患者的CD4+CD29+T细胞、IFN-γ及IL-4均较day3有显著性差异(P < 0.05)。相关性分析提示CD4+CD29+T细胞及IL-4均与Frankel分级具有显著正相关性, 而IFN-γ与Frankel分级呈显著负相关性。结论CD4+CD29+辅助性T细胞及IL-4升高, 而IFN-γ降低是脊髓损伤的重要免疫特征, 与病情转归相关, 且与损伤程度呈相关性, 因此具有重要临床意义。

The expression and clinical significances of CD4+CD29+T cells for patients with acute spinal cord injury

ObjectiveTo explore the expression and clinical significances of CD4+CD29+T cells for patients with acute spinal cord injury (ASCI).Methodcollecting 75 cases of patients with spinal cord injury, with 50 cases of healthy people as control group, using flow cytometry test peripheral blood CD4+CD29+ helper T cells and intracellular cytokines gamma interferon (IFN-gamma) and interleukin 4(IL-4) content; In 3 and 7 days after injury, review three and analyze the correlation between the three and Frankel classification.Resultscompared with normal, spinal cord injury patients with CD4+T cells and IL-4+CD29 content increased significantly, but the content of IFN-gamma significantly decreased (P < 0.05);Treatment for 3 days, CD4+T cells and IL-4+CD29 is injury to some extent on day 1, and IFN-gamma recovery in different level, of which the change of D and E significantly (P < 0.05).Treatment for 7 days, CD4+T cells and IL-4+CD29 continue to fall, IFN-gamma continue to rebound, D and E+T cells in patients with CD4+CD29, IFN-gamma and IL-4 than day3 have significant difference (P < 0.05).Correlation analysis suggests CD4+T cells and IL-4+CD29 with Frankel classification has significant positive correlation, and IFN-gamma and Frankel classification has significant negative correlation.ConclusionCD4+CD29+helper T cells and IL-4, and IFN-gamma reduce is one of the important immune characteristics of spinal cord injury associated with disease outcomes, and and relevance to the damage degree, therefore has important clinical significance.