寻常性银屑病患者外周血Th22细胞及其白细胞介素22的表达

目的 探讨寻常性银屑病患者外周血Th22细胞及相关因子白细胞介素22（IL-22）表达水平与疾病严重程度及病程的相关性。 方法 抽取40例寻常性银屑病患者和30例健康对照者外周血，流式细胞仪检测外周血Th22细胞比例，ELISA法检测血清中IL-22的表达。采用t检验、Pearson相关分析法进行分析。 结果 寻常性银屑病患者外周血Th22细胞百分比（0.65% ± 0.48%）高于健康对照组（0.33% ± 0.15%），两组差异有统计学意义（t = 3.89，P < 0.01）；银屑病患者Th22细胞与银屑病病情严重度评分（PASI）呈正相关（r = 0.38，P < 0.05），但与病程无相关性（r = 0.20，P > 0.05）。银屑病患者血清中IL-22表达水平［（67.96 ± 14.32） ng/L］高于健康对照组［（40.59 ± 9.91） ng/L］，两组差异有统计学意义（t = 9.45，P < 0.01）；银屑病患者血清IL-22表达水平与PASI呈正相关（r = 0.94，P < 0.01），与病程无相关性（r = 0.10，P > 0.05）。 结论 Th22细胞及IL-22在寻常性银屑病外周血中有高表达，且Th22细胞及IL-22水平均与疾病严重程度相关。

Frequency of T helper type 22 cells and expression of interleukin-22 in peripheral blood of patients with psoriasis vulgaris

Chen Yongfeng*, Xin Tiantian, Wang Xiaohua, Chen Wenjing, Cai Bishan, Xue Yaohua. *Guangdong Provincial Dermatology Hospital, Guangzhou 510091, ChinaCorresponding author: Chen Yongfeng, Email: gdcyf@163.com 【Abstract】 Objective To determine the frequency of T helper type 22 （Th22） cells and expression level of interleukin-22 （IL-22） in peripheral blood of patients with psoriasis vulgaris, and to investigate their relationship with disease severity and clinical course. Methods Peripheral blood samples were obtained from 40 patients with psoriasis vulgaris and 30 healthy human controls. Five-color flow cytometry was performed to determine the percentage of Th22 cells in peripheral blood, and enzyme-linked immunosorbent assay （ELISA） to measure the expression of serum IL-22. Statistical analysis was carried out by t test and Pearson correlation analysis. Results Both the percentage of Th22 cells and serum level of IL-22 in peripheral blood were significantly higher in patients with psoriasis vulgaris than in healthy human controls （Th22 cells: 0.65% ± 0.48% vs. 0.33% ± 0.15%, t = 3.89, P < 0.01; IL-22: （67.96 ± 14.32） vs. （40.59 ± 9.91） ng/L, t = 9.45, P < 0.01）. Further more, Th22 cell percentage and IL-22 serum level in peripheral blood were both positively correlated with psoriasis area and severity index （PASI） in these patients （r = 0.38, 0.94, P < 0.05 and 0.01, respectively）, but neither of them correlated with clinical course of psoriasis vulgaris （r = 0.20, 0.10, respectively, both P > 0.05）. Conclusions The percentage of Th22 cells and level of IL-22 are increased in peripheral blood of patients with psoriasis vulgaris, and both of them are correlated with disease severity.