饱和氢气水对大鼠视神经夹伤模型视网膜神经节细胞保护作用的实验研究

 目的 探索饱和氢气水对大鼠视神经夹伤模型视网膜神经节细胞（RGC）的保护作用。设计 实验研究。研究对象 SPF级SD大鼠18只。方法 对18只大鼠采用随机数表法随机分为3组，每组6只。均选取右眼为实验眼，左眼为正常对照眼。使用40 g微型视神经夹在大鼠视神经球后2 mm处夹持60 s建立视神经夹伤模型。A组给予饱和氢气水腹腔注射，5 ml/kg，每日1次；B组和C组分别给予饱和氢气水和生理盐水滴眼，每次1滴，每日3次。用药第9天，麻醉下采用3%荧光金双上丘两点注射法逆行标记大鼠RGC，第14天深麻醉下取眼球并处死动物，行视网膜定向铺片，距离视乳头中心上下左右各2 mm 拍摄照片，盲法计数RGC。主要指标 RGC存活率。结果 A组、B组和C组RGC存活率分别为40.35%±13.04%、58.34%±14.00%和43.07%±7.80%（F=3.965, P=0.041）。其中B组与A组和C组之间均有显著性差异（P=0.020；P=0.042）；A组和C组之间无显著性差异（P=0.698）。结论 饱和氢气水滴眼2周对大鼠视神经夹伤模型视网膜神经节细胞可能具有一定的保护作用。（眼科，2014, 23： 107-110）

Neuroprotective effects of saturated hydrogen water on retinal ganglion cells in rat optic nerve crush model

Objective To investigate the protective effect of saturated hydrogen water on the retinal ganglion cells (RGC) of the rat optic nerve crush model. Design Experimental study. Participants Eighteen healthy adult male SD rats. Methods Eighteen SD rats were randomly divided into the A, B and C group. There were 6 rats in each group. All the right eyes were selected as the experimental eyes, the left eyes served as controls. And the optic nerve crush model was established by using a micro optic nerve forceps with the clipping pressure of 40 g to crush the exposed optic nerve for 60 s at 2 mm from the optic disc. The rats of group A were given saturated hydrogen water by intraperitoneal injection with a dose of 5 ml/kg/d； those of group B were given saturated hydrogen water by dropping three times a day; and those of group C were given saline by dropping three times a day for consecutive two weeks. At the 9th days after injury, 3% fluorogold injection to both superior colliculus for labeling RGC under anesthesia. At the 14th days after injury, the rats were sacrificed, and RGCs were photographed and counted with mask method. Main Outcome Measures Survival percentages of RGCs. Results The average survival percentage of RGCs in A, B and C group was 40.35%±13.04%, 58.34%±14.00%, and 43.07%±7.80%, respectively（F=3.965, P=0.041）. A significant difference was found between group B and A（P=0.020）, between group B and C（P=0.042）. The difference between group A and C was no significant（P=0.698）. Conclusion Dropping saturated hydrogen water into eyes for 14 days may play a protective role for RGCs in rat optic nerve crush model. (Ophthalmol CHN, 2014, 23: 107-110)