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| 低氧对牙周膜成纤维细胞增殖和成骨分化的影响 | |
| 引用本文： | 董家辰,宋忠臣,束蓉,李松,林智恺,张秀丽. 低氧对牙周膜成纤维细胞增殖和成骨分化的影响[J]. 上海口腔医学, 2014, 23(4): 397-401 |
| 作者姓名： | 董家辰 宋忠臣 束蓉 李松 林智恺 张秀丽 |
| 作者单位： | 1.上海交通大学医学院附属第九人民医院·口腔医学院 牙周病科; 2.上海市口腔医学研究所,上海市口腔医学重点实验室,上海 200011 |
| 基金项目： | 国家自然科学基金(81070838,81271156); 上海交通大学医工交叉研究基金(YG2011MS31) |
| 摘 要： | 目的:观察低氧对体外培养的人牙周膜成纤维细胞(periodontal ligament cells, PDLCs)增殖与成骨分化的影响。方法:体外培养鉴定人牙周膜成纤维细胞,分别用浓度为0、100、200、400 μmol/L的CoCl2作用于细胞,采用MTT法观察CoCl2对PDLCs增殖的影响,运用实时定量PCR和Western免疫印迹技术检测CoCl2模拟低氧对PDLCs成骨分化的影响。采用SPSS13.0软件包对数据进行统计学分析。结果:免疫组化染色结果证实,培养细胞为牙周膜成纤维细胞。200 μmol/L及400 μmol/L的CoCl2均能抑制PDLCs的增殖及碱性磷酸酶、RUNX2、Ⅰ型胶原的表达,并呈剂量依赖性。结论:氯化钴模拟的低氧环境对PDLCs增殖及成骨分化具有抑制作用。 |
| 关 键 词： | 牙周膜成纤维细胞 低氧 氯化钴 成骨分化 |
| 收稿时间： | 2013-11-29 |
The effect of hypoxia on proliferation and osteogenic differentiation of periodontal ligament cells |
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| DONG Jia-chen,SONG Zhong-chen,SHU Rong,LI Song,LIN Zhi-kai,ZHANG Xiu-li. The effect of hypoxia on proliferation and osteogenic differentiation of periodontal ligament cells[J]. Shanghai journal of stomatology, 2014, 23(4): 397-401 | |
| Authors: | DONG Jia-chen SONG Zhong-chen SHU Rong LI Song LIN Zhi-kai ZHANG Xiu-li |
| Affiliation: | 1.Department of Periodontology, Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine. Shanghai 200011; 2.Shanghai Research Institute of Stomatology, Shanghai Key Lab of Stomatology. Shanghai 200011, China |
| Abstract: | PURPOSE: To investigate the effect of hypoxia on proliferation and osteogenic differentiation of periodontal ligament cells (PDLCs) in vitro. METHODS: Human PDLCs were isolated and characterized. The proliferation rate of PDLCs under different concentration of CoCl2 were tested by MTT assay. The PDLCs’ osteogenic differentiation were investigated using real-time PCR and Western blot. The date was statistically analyzed with SPSS13.0 software package. RESULTS: Immunocytochemical staining verified that the isolated cells were PDLCs. The proliferation of PDLCs and the expression of alkaline phosphatase (ALP), RUNX2, collagen I were significantly decreased in a dose-dependent manner by 200 μmol/L CoCl2 and 400μmol/L CoCl2. CONCLUSIONS: Hypoxia inhibits proliferation and osteogenic differentiation of human PDLCs. |
| Keywords: | Periodontal ligament cells Hypoxia Cobalt chloride Osteogenic differentiation |
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