血清炎症细胞因子与妊娠期糖尿病胰岛素抵抗的相关性研究

目的探讨血清炎症细胞因子与妊娠期糖尿病胰岛素抵抗的关系。方法分别采用放免法和ELISA法测定110例妊娠期糖尿病患者（GDM组）和60例正常孕妇（对照组）血清白细胞介素-6（IL_6）及肿瘤坏死因子-α（TNF-α）水平，采用胶乳增强免疫透射比浊法检测两组患者血清高敏C-反应蛋白（hs—CRP）水平，并计算胰岛素抵抗指数（HOMA—IR）。结果GDM组的m清IL-6、TNF—α和hs—CRP水平及HOMA—IR明显高于对照组，差异有统计学意义，均P〈0．01（t=13．088，11．980，18．900，9．801）；GDM组患者血清IL-6水平与HOMA—IR呈显著正相关（1=0．389，P〈0．01）；TNF—α水平与HOMA—IR呈显著正相关（γ=0．582．P〈0．01）；hsCRP与IR呈显著正相关（γ=0．323，P〈0．01）。结论血清炎症细胞因子与妊娠期糖尿病患者胰岛素抵抗密切相关，可能参与了妊娠期糖尿病的发生、发展。

Association between Serum Inflammatory Cytokines and Insulin Resistance in Gestational Diabetes Mellitus

Objective To investigate the relationship between serum inflammatory, cytokines and insulin resistance in gestational diabetes mellitus. Methods Radio-immunity and ELISA methods were used to measure the serum levels of interleukin-6（IL-6） and tumor necrosis factor-alpha（TNF-α ） between 110 cases of gestational diabetes mellitus（GDM group） and 60 healthy pregnant women （control group） respectively; latex-enhanced immunoturbidimetric method was used for the measurement of serum high-sensitivity C-reactive protein（hsCRP） and HOMA-IR was calculated. Results The levels of serum IL-6, TNF-α ,hs-CRP and HOMA-IR were markedly higher in the GDM group than in the control group, showing signficant differences between two groups, all P〈0.01（t =13.088,11.980,18.900,9.801 repectively）. In the GDM group, HOMA-IR was directly related to the serum IL-6（γ=0.389,P〈0.01） and TNF-α （γ=0.582,P〈0.01） as well as hsCRP （γ=0.323,P〈0.01）. Conclusion Serum inflammatory cytokines prove to be associated with insulin resistance in gestational diabetes mellitus, and may participate in the development and progress of the GDM.