HPV16-E6-DNA病毒载量对预测宫颈癌患者生存状况的影响

目的：探讨宫颈癌组织中人乳头瘤病毒16型致癌基因E6（HPV16-E6-DNA）的病毒载量对预测宫颈癌患者生存状况的影响。方法选取126例宫颈癌患者为研究对象，提取宫颈癌组织DNA，利用荧光定量PCR技术检测宫颈癌组织中HPV16-E6-DNA病毒载量，根据检测的HPV16-E6-DNA载量将患者分为低载量组（﹤106 copy/ng）与高载量组（≥106 copy/ng）。采用Kaplan-Meier法计算两组患者的生存率，采用Cox回归对影响患者5年生存率的因素进行分析。结果比较两组患者间一般资料特征，年龄、FIGO分期及淋巴结转移情况，差异有统计学意义（P﹤0.05）。Kaplan-Meier生存率分析显示，低载量组患者1年、3年及5年生存率分别为95.67%、89.32%和74.29%，高载量组患者1年、3年及5年生存率分别为89.09%、62.60%和51.37%，低载量组高于高载量组，两组差异均有统计学意义（P﹤0.01）。Cox多因素回归分析结果显示，HPV16-E6-DNA病毒载量及淋巴结转移是影响宫颈癌患者预后的主要因素。结论 HPV16-E6-DNA病毒载量与宫颈癌患者的远期预后密切相关，可作为术后宫颈癌患者预后的预测因子。

The effect of HPV16-E6-DNA viral load on the survival of patients with cervical cancer

Objective To investigate the predictive value of oncogenes E6 of human papillomavirus type 16 (HPV16-E6-DNA) on the survival of patients with cervical cancer. Method 126 patients with cervical cancer were included in the study, and the cervical cancer DNA were extracted from cervical tissues. Viral load of HPV16-E6-DNA were detected by fluorescence quantitative PCR, and then patients were stratified as low load group (HPV16-E6-DNA<106 copy/ng) and high load group (HPV16-E6-DNA≥106 copy/ng) according to the PCR results. Survival rate was calculated by Ka-plan-Meier, and the factors affecting the 5-year survival were analyzed by Cox regression analysis. Result Age, FIGO stage and lymph node metastasis in the two groups were statistically different, which were of statistically significance (P<0.05). Kaplan-Meier survival analysis showed that, 1-, 3- and 5-year survival rates of low load group were 95.67%, 89.32%and 74.29%, and were 89.09%, 62.60%, 51.37%in high load group, respectively, and the difference was statisti-cally significant (P<0.01). Cox multivariate regression analysis showed that, HPV16-E6-DNA viral load and lymph node metastasis were the main prognostic factors in patients with cervical cancer. Conclusion HPV16-E6-DNA viral load was closely associated with the long-term prognosis of patients with cervical cancer, which is useful in predicting the postoper-ative prognosis in those patients.