JNK抑制剂CEP-1347对噪声损伤耳蜗的保护作用及意义

目的 探讨JNK抑制剂CEP-1347对噪声损伤耳蜗的保护作用及意义。方法 27只wistar大鼠随机分成3大组，A组：噪声组，B组：噪声＋CEP 1347组，C组：噪声＋盐水组，分别给予噪声暴露，B组在噪声暴露前给予皮下注射CEP-1347；C组同时间皮下注射等量生理盐水。在噪声暴露后的1、4及14d分别测试各组的ABR阈值，同时检测外毛细胞的凋亡情况。结果 ABR阈值及外毛细胞凋亡数显示B组在1d时与A组比较差异无统计学意义（P>0.05），4d时听力较A组差异有统计学意义（P<0.05），用药后第14d听力与A组比较差异有统计学意义（P<0.01）；C组在各时间点与A组比较差异无统计学意义（P>0.05）。结论 应用JNK抑制剂CEP-1347进行干涉，可以减轻噪声造成的听力损失，JNK抑制剂的开发可能会成为NIHL防治的一个新的靶点和方向。

Protection of CEP-1347 on noise-damaged cochlea

Objective To explore the protective effect and implications of JNK inhibitor CEP-1347 on noise-damaged cochlea. Methods Twenty seven wista r rats were divided randomly into three major groups: group A, the noise group; group B, the noise plus CEP-1347 group; group C, the noise plus saline group. All rats were exposed to noise. Group B was subcutaneously injected with CEP-1347 while group C was injected with same dose saline before the exposure. The ABR threshold and apoptosis of outer hair cell were detected on the first, fourth and 14thday after exposure in all groups. Results ABR threshold and apoptosis counts showed no statistical significance between group B and A on the first day (P>0.05). However, significant differences appeared on day 4, (P<0.05) and day 14 (P<0.01). There was no statistical significance between group C and A during the whole time frame(P>0.05). Conclusion Inhibitor of JNK pathway, CEP-1347, may alleviate the noise-induced apoptosis and hearing loss to some extent. JNK inhabitor may serve as a new target and subject for NIHL research.