两种植物挥发油体外抗菌作用试验观察

目的观察金银花和山楂果核挥发油体外抗菌效果,为实际应用提供科学依据。方法采用稀释法对两种植物挥发油体外最小抑菌浓度和最小杀菌浓度进行了测定。结果金银花挥发油对革兰阴性菌株的MIC50和MIC90值均≥128μg/ml,对金黄色葡萄球菌MIC90值≥128μg/ml、MIC50值为64μg/ml。山楂果核挥发油对所有试验菌株的MIC50值在16～64μg/ml范围;MIC90值在64～128μg/ml。山楂果核挥发油作用15 min,对大肠杆菌和金黄色葡萄球菌MBC值在32～64μg/ml范围;金银花挥发油作用60 min,对上述两种细菌MBC值为64μg/ml。结论两种植物挥发油对试验菌株都具有良好的体外抗菌效果,但其MIC和MBC值均明显高于两种对照药物。

OBSERVATION ON ANTIBACTERIAL EFFECT IN VITRO OF TWO KINDS OF PLANT VOLATILE OIL

Objective To observe the antibacterial effect in vitro of volatile oil from honeysuckle and hawthorn fruit stone in order to provide a scientific basis for practical application.Methods The minimum inhibitory concentration test and minimum bactericidal concentration test of each volatile oil were used.Results The MIC50 and MIC90 values of volatile oil from honeysuckle against Gram-negative bacteria strains were ≥128 μg/ml;the MIC90 was ≥128 μg/ml and the MIC50was 64 μg/ml against Staphylococcus aureus.The MIC50 values of volatile oil from hawthorn fruit stone against all strains were 16-64 μg/ml and the MIC90 values were 64-128 μg/ml.Results of the MBC showed that the volatile oil from hawthorn fruit stone for 32-64 μg/ml could kill Staphylococcus aureus and Escherichia coli exposed for 15 min while the volatile oil from honeysuckle for 64 mg/ml could kill the bacteria above exposed for 60 min.Conclusion The two kinds of tested volatile oil have good germicidal effect in vitro,but the MIC and MBC values are remarkably higher than those of two controlled drugs.