超高效液相色谱法同时测定白芍中芍药苷和芍药内酯苷的含量

目的 采用超高效液相色谱法(UPLC)同时测定白芍中芍药苷和芍药内酯苷的含量.方法 采用Agilent Extend-C18(4.6 mm×50 mm,1.8μm)色谱柱,以流动相乙腈(A)-0.2％乙酸溶液(B)梯度洗脱；柱温30℃；流速0.5 mL·min-1；检测波长230 nm.结果 芍药苷和芍药内酯苷分别在0.011 6～1.45(r=0.999 9)、0.008～lmg·mL-1(r=0.999 9)呈良好的线性关系；平均回收率分别为98.6％、97.5％,RSD分别为1.4％、2.2％.结论 该法快速简便,灵敏度高,准确可靠,可用于白芍中有效成分含量的快速测定及白芍的质量控制.

Simultaneous determination of paeoniflorin and albiflorin in Radix Paeoniae Alba by UPLC

Objective To establish a UPLC method for the simultaneous determination of paeoniflorin and albiflorin in Radix Paeoniae Alba.Methods Agilent Extend-C18(4.6 mm×50 mm,1.8 μm) was used with acetonitrile(A)-0.2% acetic acid solution(B)as the mobile phase in gradient elution0-2.5 min,8%-12%A;2.5-10 min,12%-20%A].The flow rate was 0.5 mL·min-1,the column temperature was 30℃,and the detection wavelength was 230 nm.Results The linear ranges of paeoniflorin and albiflorin were 0.011 6-1.45(r=0.999 9) and 0.008-1 mg·mL-1(r=0.999 9);the average recoveries were 98.6% and 97.5%;and the RSDs were 1.4% and 2.2%.Conclusion The method is rapid,sensitive,and reliable,which can be used for the quality control of Radix Paeoniae Alba.