塞来昔布对人肺癌A549细胞株增殖抑制和凋亡诱导作用观察

目的 体外研究选择性环氧化酶2 (COX-2)抑制剂塞来昔布对人肺癌细胞株A549增殖和凋亡的影响,并探讨其可能的作用机制.方法 采用噻唑蓝(MTT比色法)观察不同浓度的塞来昔布对人肺癌细胞株A549的增殖抑制作用,TUNEL染色检测细胞凋亡,并用含半胱氨酸的门冬氨酸蛋白水解酶-3(caspase-3)活性检测试剂盒检测其活性变化.结果 塞来昔布能够以浓度依赖性方式有效地抑制K562细胞增殖,药物的Ic50为33.98 μmol·L-1;TUNEL染色分析显示给予不同浓度塞来昔布的细胞与未给予塞来昔布的细胞相比凋亡率有差异(P＜0.05),经塞来昔布处理的A549细胞内caspase-3的A405较正常对照组有显著增加(P＜0.05).结论 塞来昔布能够抑制A549细胞增殖,并呈药物浓度依赖性；塞来昔布能以浓度依赖性方式诱导K562细胞凋亡,其机制涉及caspase-3活化的信号转导途径.

Celecoxib-induced anti-proliferation and apoptosis of human lung adenocarcinoma cell line A549

Objective To investigate the effect of celecoxib,a selective COX-2 inhibitor,on human lung adenocarcinoma cell line A549,and to explore its molecular mechanism.Methods After incubating K562 cells with increasing dose of celecoxib in vitro,the cell viability was analyzed with MTT assay,apoptosis of cells was detected by TUNEL,and caspase-3 activity was detected by caspase-3 activity detection kit.Results Celecoxib effectively inhibited the proliferation of A549 cells in a concentration-dependent way,and the IC50 of A549 cells was 33.98 μmol·L-1.TUNEL assay indicated that the rate of apoptosis was significantly different in cells with or without celecoxib(P<0.05).The cellular caspase-3 A405 of A549 cells treated with celecoxib was obviously higher than that of the control group(P<0.05).Conclusion Celecoxib can inhibit the proliferation and induce the apoptosis of A549 cells in a concentration-dependent manner.The regulation of apoptosis induction is related to the caspase-3-mediated pathway.