| HPLC-ELSD法测定消栓通络胶囊中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的含量 |
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| 引用本文： | 曾岗,李慧敏.HPLC-ELSD法测定消栓通络胶囊中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的含量[J].中南药学,2012,10(1):16-19. |
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| 作者姓名： | 曾岗 李慧敏 |
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| 作者单位： | 1. 华容县人民医院,湖南岳阳,414200
2. 岳阳市药品检验所,湖南岳阳,414000 |
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| 摘 要： | 目的 采用高效液相色谱蒸发光散射检测器(HPLC-ELSD)法测定消栓通络胶囊中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的含量.方法 以乙腈-水为流动相梯度洗脱(0～12 min,水80％-65％),Hypersil ODS柱(4.6 mm×250 mm,5 μm)为固定相,流速为1.0 mL·min-1,ELSD参数:漂移管温度:45℃,载气流量:1.5L·min1.结果 三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的回收率分别为96.7、96.4％和97.7％,RSD分别为1.6％、1.4％和2.2％.结论 该方法简便、快速、重现性好,可用于消栓通络胶囊的质量控制.
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| 关 键 词： | 消栓通络胶囊 高效液相色谱-蒸发光散射检测器 三七皂苷R1 人参皂苷Rg1 人参皂苷Rb1 |
Determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Xiaoshuan Tongluo Jiaonang by HPLC-ELSD |
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| ZENG Gang
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LI Hui min.Determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Xiaoshuan Tongluo Jiaonang by HPLC-ELSD[J].Central South Pharmacy,2012,10(1):16-19. |
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| Authors: | ZENG Gang LI Hui min |
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| Institution: | 2(People’s Hospital of Huarong,Yueyang Hunan 414200;2.Yueyang Institute for Drug Control,Yueyang Hunan 414000) |
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| Abstract: | Objective To develop an HPLC-ELSD method for the content determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Xiaoshuan Tongluo Jiaonang. Methods Hypersil ODS analytical column(4.6 mm×250 mm,5 μm) was used with the mobile phase consisting of acetonitrile-water in gradient elution(0-12 min,water 80%→65%).The detector drift tube temperature was 45 ℃ and the nitrogen flow rate was 1.5 L·min-1. Results The recoveries for notoginsenoside R1,ginsenoside Rg1,and ginsenoside Rb1 were 96.7%,96.4%,and 97.7% with RSD 1.6%,1.4%,and 2.2%,respectively. Conclusion The method is simple,rapid,repeatable,and can be used for the quality control of Xiaoshuan Tongluo Jiaonang. |
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| Keywords: | Xiaoshuan Tongluo Jiaonang HPLC-ELSD notoginsenoside R1 ginsenoside Rg1 ginsenoside Rb1 |
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