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| 全新丹参素衍生物抗心肌细胞凋亡作用研究 | |
| 引用本文： | 赵宏苏,张名娟,许本洪,郭琳,傅和亮,单璐琛.全新丹参素衍生物抗心肌细胞凋亡作用研究[J].中南药学,2012,10(6):418-421. |
| 作者姓名： | 赵宏苏 张名娟 许本洪 郭琳 傅和亮 单璐琛 |
| 作者单位： | 1. 暨南大学药学院新药研究所,广州,510632 2. 广州科技职业技术学院,广州,510550 3. 广东华南新药创制中心,广州,510663 |
| 基金项目： | 暨南大学科研培育与创新新基金(No.11611318) |
| 摘 要： | 目的 研究全新丹参素衍生物ADTM对心肌细胞凋亡的影响.方法 体外培养H9c2心肌细胞,t-BHP诱导H9c2细胞凋亡,Hoechst染色观察ADTM对心肌细胞凋亡的影响；结扎大鼠左冠状动脉前降支建立大鼠心肌缺血模型,缺血24 h后提取心肌组织蛋白,Western blot方法检测心肌组织凋亡相关蛋白Bcl-2、Bax以及caspase-3的表达.结果 ADTM能显著抑制t-BHP诱导的H9c2心肌细胞凋亡；在大鼠心肌缺血模型中,明显提高Bcl-2蛋白的表达,降低Bax和caspase-3蛋白的表达,减少缺血心肌细胞凋亡.结论 ADTM可显著抑制心肌细胞凋亡,从而减轻心肌细胞缺血损伤. |
| 关 键 词： | 丹参素衍生物 心肌缺血 心肌细胞凋亡 |
Anti-apoptosis effect of a novel derivative of Danshensu on cardiomyocytes |
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| ZHAO Hong-su , ZHANG Ming-juan , XU Ben-hong , GUO Lin , FU He-liang , SHAN Lu-chen.Anti-apoptosis effect of a novel derivative of Danshensu on cardiomyocytes[J].Central South Pharmacy,2012,10(6):418-421. | |
| Authors: | ZHAO Hong-su ZHANG Ming-juan XU Ben-hong GUO Lin FU He-liang SHAN Lu-chen |
| Institution: | 1(1.Institute of New Drug Research of Jinan University,Guangzhou 510632;2.Guangzhou Vocational College of Science and Technology,Guangzhou 510550;3.South China Center for Innovative Pharmaceuticals,Guangzhou 510663) |
| Abstract: | Objective To investigate the in vitro and in vivo anti-apoptosis effect of ADTM,a novel derivative of Danshensu,on cardiomyocytes.Methods Hoechst 33258 staining was used to detect H9c2 cell apoptosis induced by t-BHP in vitro experiment.A rat model of acute myocardial ischemia was established by the ligation of left coronary artery anterior descending branch,and the expression of apoptosis-related protein Bcl-2,Bax and caspase-3 was measured by Western blot.Results ADTM significantly inhibited the H9c2 cell apoptosis.ADTM up-regulated the expression of Bcl-2 protein and down-regulated the expression of Bax and caspase-3 in a dose-dependent manner.The biological activity of ADTM was remarkably better than Danshensu.Conclusion Inhibition of cardiomyocytes apoptosis may be a potential protective mechanism of ADTM against cardial ischemia injury. |
| Keywords: | derivative of Danshensu myocardial ischemia cardiomyocyte apoptosis |
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